Site-directed mutagenesis of a lytic polysaccharide monooxygenase from Micromonospora aurantiaca

(D) Structures of the possible single FITC labelled products arising from incomplete labelling, with theoretical masses and the observed fragmentation patterns (using the

Although the common protein fold of LPMOs indicates that heterologous expression of these proteins is likely to succeed in the most common bacterial and fungal expression hosts,

Herein, we compare two processive chitinases, ChiA and ChiB, one mutant, ChiB-W97A, and the endochitinase ChiC of the well-characterized chitinolytic machinery of Serratia

At 10 mM metal ion concentration, Co 2+ inhibited CBP21 activity at all preincubation times, whereas samples containing 10mM Zn 2+ ions only inhibited CBP21 when the

Kracher D, Andlar M, Furtmuller PG, Ludwig R: Active-site copper reduction promotes substrate binding of fungal lytic polysaccharide monooxygenase and reduces stability. Hangasky

Indeed, studies of processive chitinases (Horn et al., 2006, Zakariassen et al., 2009) have shown that replacement of aromatic amino acids by alanine led to

Lytic polysaccharide monooxygenases (LPMOs) catalyze the oxidative cleavage of glycosidic 21. bonds and represent a promising resource for development of industrial

Please note that the lower levels of H 2 O 2 in reaction mixtures that contain substrates that are cleaved by the enzyme (Glc 5 and Glc 6 ) do not necessarily indicate that H 2