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2. THE THEORY OF ANOMALOUS DISPERSION

In document The refractive index of phytoplankton (sider 44-63)

In 1875 HELMHOLTZ presented a theory which gave a relation between the absorptions bands and the anomalous dispersion of the refractive index. His theory was based on a mechanical analogy. For the free "ether11 he assumed that the equation of motion could be written

a2t. a2~

p , = a

at 2 ay2

p is the 11density11 of "ether11 3 and a is an elasticity constant per volume unit of the ether, ~ is the dis~

placement along the y axis of a parcel of ether from its mean position. If the motion has the character of a free]

progressive harmonic wave along the y axis, it may be de~

scribed by

~

=

i;o e

i 27~( y-et)

A

1;0 is then the amplitude of the wave, A is the

wave-length and c the phase velocity. Incerting eq. 41 in 1, we obtain the relation

c2

=

a/p

When molecules are present, HELMHOLTZ thought that there are a Hook type of elastic force between each atom of the molecule and the ether, that is of the form

where S is the Hook constant, and x is the displace-ment of the atoms from their mean position. The equation of motion for the ether then becomeq

(40)

(41)

(42)

-44

-= (43)

If there are j different types of atoms present, the equation should read

=

(44)

For the j-th type atbms with mass r.

J the equation of motion is

a

2 x. ax.

r. J ~at

2

=-f.x. J J + B·(~-x.) J J - g . J ~tJ o (45)

The first term on the right side of the equation is the intermolecular Hook force which results from the dis-placement of the atom within the molecule. The molecule as a whole is supposed to be at rest. The second term is the reactio of the actio in eq. 44, and the third term describes the dissipation of energy, which is supposed to be proportional with the velocity of the oscillating atoms.

A light wave passing through this medium must then have a t~rm which accounts for the dissipation or absorption effect. Instead of eq.

41

we should write

i 29-TI ( y-vt ) ~ ~ y

~o e

and x should be of the form

X.

=

J

i 2

9-TI (y-vt )- ~ y x. e

JO

1 and v are the wavelength and velocity of the wave in this medium~ and a/2 is the absorption coefficient of the amplitude. (Since the energy is proportional with

(46)

(47)

-45

-the squax·e of the amplitude, the absorption coefficient of the energy is then a).

The exponents of the eqs. 46-47 may be written

· 27T((l aR-) - v·t).

l T +l1f7fY

By introducing the refractive index of the medium relative to ether

n

=

c v :::

and the absorption index

K ::: at.

1f7f

the exponent becomes

i ~7T ((n + iK)y- ct).

The complex refractive index m may in this context be defined as

m

=

n + iK

(The phase in eq. 46 may just as well be expressed by vt - y instead of y - vt. m will then be defined as

n - iK.

.

It should also be noted that both n and K are indices relative to the refractive index of the medium in which t. is the wavelength). The eqs. 46-47 now become

. 27T ( )

1

T

my - ct

X.::: X· e J JO

Incerting these functions in eqs. 44-45, we obtain

(48)

(49)

(50)

(51)

(52)

(53)

-

46-~rj(2>.'!!_ '--I ~-1 2 x. = -fjxjo + ej<~o-xjo) + ig. T 21T c

JO J JO

The last equation may be solved for ·X. '

JO and substituting this into eq. 53~ the result becomes after some ordering

The first term on the right side of eq. 55 is 1, according to eq. 42.

The second term is a constant,

multiplied with >.2 . It will make m2 decrease mono-tonously as >. increases~ and consequently does not de-scribe the phenomenon of anomalous dispersion. Probably the term is very small or is compensated for by the third term, otherwise m2

would soon aquire very unreasonable values outside the absorption bands, due to the >.2 in-fluence. We shall then omit this term here.

The third term gives rise to the anomalous be-haviour of m2 . If we assume that the influence of each j-contribution is restricted to a very small wavelength interval, so that the factor >.2 before the E sign may be regarded as a constant for each j-terms we may as a first approximation write

A. J

=

It will also be convenient to write

A. 2 2 2

= 41T c r./(13 .+f.)

J J J J

and

G.

=

21TC g./(f3.+f.)

J J J J

(54)

(55)

(56)

(57)

(58)

Eq. 55 then becomes

2 2 .

A. -;\. ~ 1.A.G.

J J

This equation may be separated into its real and imaginary parts:

2 2

n - K = l + 2:

2nK

=

These are the equations presented by JENKINS and WHITE (1957, p. 476) as resulting from the theory of HELMHOLTZ.

An interesting point is that a later model by LORENTZ (1915), based on electromagnetic theory, gives a formula of the type

= 2 ?

I. -A."'~ iA.G.

J J

(Lor..:;;:\!'l'Z gives the formula explicit for a non~-absorbing

mix::·cre (pae;e 311, note 58)). If the relative variation o+~ .112~--1 is much greater than the relative variation of

.-.

c: . t:h t .

m ~.~ ~ v a J..s) m is close to 1 , tl 1en m2 +2 ::: 3 and eqs. 59 and 62 become of identical form.

(59)

(60)

(61)

(62)

The indices n and K may be found from eqs. 60-61, but t.;he resulting expressions are complicated. To simplify

tJ-;.:- '1iscussion we shall just look at the behaviour around

on~ of the absorption bands. We will further ass~me that the D i.~her bands contrib-ute to a rather constant refractive index N outside the actual bandl and that their absorption

- 48

-is pl'a·.;\.;ically zero at these wavelengths. The eqs. 60-61 then become

(63)

2nK

=

(64)

From eq. 64 it is seen that if n is fairly constant~ K

will have its greatest values around A1• Wb shall now again make use of the earlier assumption that the influence of the absorption is restricted to a small interval, so that A may be regarded as constant compared with (A-A

1), and write

A2 - A 2

1 = (A - A

1)(A + A

2

)~(A - A1)2X1 (65)

A2 ~ A 2 (66)

1

A3 ~ ), 1 3 (67)

Further we shall assume that the absorption index K is small compared with the refractive index n, but that its relative variation is greater, that is

K << n ~ N (68)

I~ ~~I

>>

I~ ~I

(69)

vJith these assumptions eqs. 63-64 may be written

(70)

2NK ~ (71)

-

49-We mus· ::;:i.mplify eq. 70 further by observing that n 2-N2

=

(n-N)(n+N)

=

(n-N)(2N)

If we write B = A/2N eqs. 70~71 become

n z N +

K ~ ~

These expressions are more suitable for discussions than eqs. 60-61.

We see from eq. 75 that K will have its peak value Kl for A

=

A.l:

Kl

= ---a

A.1B

Solving for B and substituting the expression for B in Rq. 75 gives

K :

4(11.-:\1)2 + G2

It should be noted that K is a symmetric function around J.

1.

There are two wavelengths 11. 2 where K has the value K1/2. From

=

it is obtained that these wavelengths are

G 2

(72)

(73)

( 711)

(75)

(76)

(77)

(78)

(79)

-50

-Thus G is the width of the absorption band where K

has half of its peak value.

With the substitution of B from eq. 76, eq. 74 becomes

n

=

It is seen that n - N is antisymmetric around A = A1. For A

=

A1, n - N

=

0, or n ~ N.

By differentiating the last equation with regard to A~ one obtains

dn

=

dA

This expression is zero for

±

2

G

that is for A

=

A2 At these wavelengths eq. 74 gives that

K

n

=

N ± 21

The approximate model described by eqs. 74-75 thus gives that n will have a minimum value at

cross its mean value N at A1, and obtain its maximum

' G

at /\1 + 2' The difference between the maximum and minimum value of the refractive index equals the peak value of the absorption curve.

An interesting point is that if we have just one absorption band, and we are far from it, at the long wave -length side~ then

(80)

(81)

(82)

(83)

-

51-\ >> .Al

and eq. 60 may be written

which is identical with the SELLMEIER equation presented in Chapter

7.

(84) (85)

(86)

-52-REFERENCES

ALLISOl~~ R.V., 1924. The density of unicellular organisms.

Ann. appl. Biol. 11: -153-168.

ANTIA, N.J., ·MtALLISTER, C.D., PARSONS, T.R., STEPHENS, K.

and S~RICKLAND, J.H.D., 1963. Further measurements of primary production using a large-volume plastic sphere. Limn. Oceanpgr~.- ~: 166-183.

ARIVISTRONG, S.H., Jr., BUDKA, M.J.E., f!J:ORRISON,K.C. and

-HASSQ~, M., 1947. Preparation and properties of serum and plasma proteins. XII. The refractive properties of the proteins of human plasma and cer-tain purified fractions. LT. Am. Chern. Soc. 69:

1747-1753.

ATKINS, W.R.G., 1923. The phosphate content of fresh and salt waters in its relationship to the growth of the algal plankton. J. Mar. Biol. A8s. U.K. 13 (N.S.): - . 119-150.

BARER, R., 1966. Phase contrast and interference microscopy in cytology. In: Physical techniques in biological research. 2. ed. Vol.3, Part A. Cells and tissues.

Editor A.W. POLLISTER. Acad. Press. New York: 1-56.

BONEY, A.D., 1975. Phytoplankton. E. Arnold, London. 116 pp.

BORN, M. and WOLF, E., 1975. Principles of optics. 5. ed.

Pergamon, Oxford, 808 pp.

Bf:t".NDT, K. and RABEN, E., 1920. Zur Kenntnis der chemischen

Zusammensetzung des Planktons und einiger Boden-organismen. Wiss. Meeresuntersuch. Abt. Kiel, N.F.

19: 175-210.

BROWN, O.B. and GORDON, H.R., 1973a. Two component Mie-scattering models of Sargasso Sea particles. Appl.

Opt., 12: 2461-2465.

BROVJN, O.B. and GORDON, H.E., 1973b. Comment on "Method for the determination of the index of refraction of particles suspended in the ocean!1 J. Opt. Soc.

Am . , 6 3 : 1616-161 '7 •

(

-53-BRYANT, F.D., SEIBER, B.A. and LATIMER, P., 1969. Absolute optical cross sections of cells and chloroplasts.

Arch. Biochem. Biophys., 135: 97-108.

BURT, W.V., 1955. Interpretation of spectrophotometer re a-dings on Chesapeake Bay waters. J. Mar. Res.,

li:

33-Ll6

CARDER, K.L., TOMLINSON, R.D. and BEARDSLEY, G.F. Jr., 1972.

A technique for the estimation of indices of re -fraction of marine phytoplankters. Limn. Oceanogr,, 17: 833-839.

CHAMOT, E.M. and MASON, C.w., 1944. Handbook of chemical microscopy. Vol.l, John Wiley, New York3 478 pp.

CHARNEY~ E. and BRACKETT, F.S., 1961. The spectral depen-dence of scattering from a spherical alga and its implications for the state of organization of the light-accepting pigments. Arch. Biochem. Biophys., 92: 1-12.

CLARK, J.B., 1962.Physical and mathematical tables. Revised by A.C.AITKEN and R.D. CONNOR. Oliver and Boyd, Edinburgh, 91 pp.

COLLYER, D.M. and FOGG, G.E., 1955. Studies on fat accumu-lation by algae. J. Exp. Bot.

&:

256-275.

CU5HING, D.H., HUMPHREY, G.F., BANSE, K., LAEVASTU, T., 1958.

Report of the Committee on terms and equivalents.

Rapp. Cons. Int. Expl. Mer, 144: 15-16.

:'':i~'JA, E. S. , 1950. A textbool{ of mineralogy. 4. ed. Revised

by W. FORD. John Wiley & Sons) New York. 851 pp.

DAVIES, H.G., WILKINS, M.H.F., CHAYEN, J.and 'LACOUR, L.F. , 1954. The use of the interference mircoscope to determine dry mass in living cells and as a quanti -.. tativecytochemical method. Q.J. Nicrosc. Sci., 95:

271-304.

D0UGHERTY, R.C., STRAIN, H.H.:~ SVEC, W.A., UPHAUS, R.A. and KATZ , J.J., 1966. Structure of chlorophyll c. J.Am.

Chern. Soc. 88: 5037-5038.

DUYSENS, L.N.M., 1956. The flattening of the absorption spectrum of suspensions, as compared to that of solutions. Biochem. Biophys. Acta, 19_: 1-12.

~54-EU~ER, II. v. and JANSSON, B., 1931. Beziehungen zwischen Ergosterin und Carotin. Ark. kern. miner. geol.

Band lOB. No.l7. 6 pp.

FCRSY~BE, W.E., 1954. Smithsonian physical tables. 9. ed.

Smithsoni2n Institutions Washington. 827 pp.

FREY, A., 1926. Die submikroskopische Struktur der Ze ll-membranen. Jahrb. wiss. Bot. 65: 195-223.

GIBBS, T.R.P. , Jr. , 1942. Optical methods of chemical analysis. McGraw~Hill, New York. 391 pp.

GLADSTONE~ J.H. and DALE, T.P., 1863. Researches on the

refraction, dispersion and sensitiveness of liquids.

Phil. rrrans. Roy. Soc. London, ]-53: 317-343.

GORDON, H.R. and BROWN, O.B., 1972. A theoretical model of light scattering by Sargasso Sea particulates.

Lin~n . . Qceanogr., 17: 826-832.

GRIM, J., 1939. Beobachtungen am Phytoplankton des Baden -sees (Obersee) sowie deren rechnerische Auswertung.

Int. Rev. gesamt. Hydrobiol. Hydrogr. 39: 193-315.

HARlUS, E. and RILEY, G. A., 19 56. Oceanography of Long

Island Sound, 1952-195!1. VIII. Chemical composition of the plankton. Bull. Bingham oceanogr. Coll. 15:

315-323.

H./',.1-''J.!-<;Y, H.W., 1937. The supply of iron to diatoms. J. !Vlar.

Biol. Ass. U.K.,~: 205-219.

HKi,!VlHOVI'Z, H., 1875. Zur 'l'heorie der anomalen Dispersion.

Ann. Phys. Chern. )-54: 582-596.

ll~)f•C\SON, R.T. and NEvJKIRK, D.D. ~ 1975. Pyridine immersion:

A t echnique for measuring the refractive index of marine particles. Proc. Soc. Phot. Opt. Instr. Eng.

64: 62-64.

IIODKINSON, J.R. , 1963. Light scattering and extinction by irregular particles larger than the wavelength. In:

Electromagnetic scattering. Editor M. KERKER.

Pergamon Press, London: 87-100.

HOLI..AND, A.C. and GAGNE, G., 1970. The scattering of pola-rized light by polydisperse systems of irregular particles. Appl. Opt.,

2=

1113-1121.

-55-HOLLAND~ A.C. and GAGNE, G., 19711 Comment on: The scat -tering of polarized light by polydisperse systems of irregular particles. 2. Appl. Opt., 10: 1173-117 4,~.

JENKINS, F.A. and WHITE, H.E., 1957. Fundamentals of optics.

3. ed. McGraw-Hill, Inc. Tokyo. 637 pp;

JERLOV, N.G., 1976. Marine optics. Elsevier, Amsterdam.

231 pp.

J0RGENSEN, C.B., 1966. Biology of suspension feeding.

Pergamon Press, Oxford. 357 pp.

KErrCHUM, B.H. and REDFIELD, A.C., 1949. Some physical and chemical characteristics of algae growth in mass culture. J. Cell. Como. Physiol. 33: .

-

281~299.

KETELAAR, J.A.A. and HANSON, E.A., 1937. Elementary cell and space group of ethyl-chlorophyllide. Nature, l:.._40: 196.

KORITNIG, S. 1962. Brechzahl bei einzelnen Wellenlangen~

Dobbel-brechung. In Landolt Bornstein: Zahlenwerte und Funktionen. II Band. 8. Teil. Optische Kon-stanten. Springer~Verlag, Berlin: 2.43-2.397.

KULLENBERG, G., 1970. A comparison between observed and computed light scattering functions. Rep. Inst.

Phys. Oceanogr.~ Univ. Copenhagen, ].3: 22 pp.

I\1:i1 ,1_,_i.:NBEHG, G. and BERG OLSEN, N. , 1972. A comparison

betvreen observed and computeC:. Iight scattering func-tions - II. Rep. Inst. Phys. Oceanogr., Univ.

Copenhagen, 19, 27 pp.

1..J:\~'TI'IIER, P. and RABINOVJITCH, E., 1959. Selective scattering of light by pigments in vivo. Arch. Biochem. Bio-phys. ~: 428-441.

LEWIN, J.C., l962a. Silicification. In: Physiology and bio-chemistry of algae. Editor R.A. LEWIN. Acad.

Press, New York: 445-455.

LEWIN, J.C., 1962b. Calcification. As above. pp. 457- 465 .

LORENTZ, H.A., 1880. Uber .... die Beziehung zwischen der Fort -pflanzungsgeschwindigkeit des Lichtes und der K6r~

perdichte. Ann. Phys. Chern. N.F . .2_: 641-·665.

-56-LGRENTZ, H.A., 1915. The theory of electrons. z.ed. Dover, New York. 339 pp.

LORENZ 9 L., 1880. Uber die Refractionsconstante. Ann.Phys.

Chemie. N.F. 11: 70-103.

McALLISTER, C.D., PARSONS, T.R., STEPHENS, K. and STRICKLANDi J.H.D., 1961. Measurements of primary production in coastal sea water using a large-volume plastic sphere.

Linm .. Oceanogr. ~: 237-259.

McCRONE, W.C., DRAFTZ, R.G. and DELLY, J.G., 1967. The particle atlas. Ann A~bor Science Publ. Ann Arbor. 406 pp.

MOREL, A., 1973. Diffusion de la lumi~re par les eaux de mer.

Resultats experimentaux et approche theorique. In:

Optics of the sea, AGARD-LS-61, NATO,

Brussels:3.1-l 76.

ONCLEY, J.L., SCATCHARD, G. and BROWN, A., 1947. Physical-chemical characteristics of the proteins of normal human plasma. J.Phys.Chem. 51: 184-198.

OSTER, G., 1955. Birefringence and dichroism. In: Physical techniques in biological research. Eds. G. OSTER and A.W. POLLISTER. Vol.l. Optical techniques. Acad.

Press. New York: 439-460.

PARSO~ T.R., 1963. Suspended organic matter in seawater. In:

Progress in oceanography9 vol.l. Editor M. SEARS.

Pergamon Press9 Oxford: 205-239.

PA~~ONS, T.R., STEPHENS, K. and STRICKLAND, J.H.D., 1961.

On the chemical composition of eleven species of ma-rine phytoplankters. J.Fish.Res.Bd. Canada, 18:

1001-1016.

PLASS, G.N. and KATTAW!\R, G.W., 1971. Comment on: The scat-tering of polarized light by polydisperse systems of irregular particles. l.Appl.Opt., 10: 1172-1173.

RABINOWITCH, E.I., 1945. Photosynthesis and related processes.

Vol.l. Interscience Publ. , New York. 599 pp.

rutr3INOWrrcH, E. I. 1 1951. Photosynthesis and related processes.

Vo1.2. Part 1. Interscience, New York: 603-1208.

Hj~LEY, G.A. 3 19111. Plankton studies. IV. Georges Bank. Bull.

Bingham Oceanogr.Coll.

1,

Art. 4, 73 pp.

ROSS, K.F.A., 1967. Phase contrast and interference micro-scopy for cell biologists. E. Arnold Ltd., London.

238 pp.

SASAKI, T., OKAMI, N., OSHIBA, G. and WATANABE, S., 1960. An-gular distribution of scattered light in deep sea \vater, Rec.Occanogr. Works. Jap.

2=

1-10.

SMAYDA, T.J., 1970. The suspension and sinking of phytoplankton in the sea. Oceanogr.Mar.Biol.Ann.Rev. ~: 353-414.

Sl'l·r~~-IR, H.R., and MILNER, H.W., 1949. The chemical composition

of Chlorella; effect of environmental conditions.

Plant Physiol. ~: 120-149.

STECHER, P.G. 5 editor, 1968. The Merck Index. 8.ed. Merck & Co., Rahway, N.J. 1713 pp.

STRICKLAND, J.H.D.} 1960. Measuring the production of marine phytoplankton. Fish.Res.Bd. Can. Bull., 122-.·J.. 172 pp.

SUGIHARA, S. and TSUDA, R., 1979. Light scattering and size distribution of particles in the surface waters of the North Pacific Ocean. J.Oceanogr.Soc.Jap.,

..2.2.:

82-90.

TIMASHEFF, S.N., 1976. Refractive index increments of proteins.

In Handbook of biochemistry and molecular biology.

3.ed. Proteins. Vol.2. Editor G.D. FASMAN. CRC, Cleveland: 372-382.

':\;':. : ;·BEE~ E. , 1955. Die Chemie der Zellwand. In: Handbuch der

Pflanzenphysiologie.

W. RUHLAND, editor. Springer-Verlag, Berlin: 668-721.

VAN DE HULST, H.C., 1957. Light scattering by small particles.

John Wiley & Sons, New York. 470 pp.

VINOGRADOV, A.P., 1953. The elementary chemical composition of marine organisms. Yale Uni v. Press, Nev.r Haven, Con-necticut. 647 pp.

;; ,_~lEBURN, E.W., editor, 1927. International Critical Tables,

Vol.II. McGraw-Hill, New York. 661 pp.

-58

-W~AST, R.C., editor, 1977. Handbook of chemistry and physics.

58. ed. CRC Press, Cleveland.

WIENER, 0., 1912. Die Theorie des Mischkorpers fUr das Feld der stationaren Stromung. Abh.Math.Phys.Kl.Konigl.

Sachsischen Ges.Wiss., 32: 507-604.

lf.'ENER, 0., 1927. Formdoppelbrechung bei Absorption. Kolloid-chem. Beih. ~: 189-198.

WOLKEN, J.J. ~ 1973. Photodynamics: The chloroplast in photo-synthesis. In: L. P. MILLER, editor: Photochemistry, Vol.l. van Nostrand Reinhold Camp. New York: 15-37.

Zr~J\i2VELD, J.R.V. and PAK, H., 1973. Method for the determina-tion of the index of refracdetermina-tion of particles suspended in the ocean. J.Opt.Soc.Am., 63: 321-324.

Zi\(mVELD, J.R.V. , ROACH, D.M. and PAK,H., 1974. The deter-mination of the index of refraction distribution of oceanic particles. J.Geophys.Res., 79: 4091-4095.

Fig.l. The .refractive index of a two-component system, based on diffe-rent equations, as a func-tion of the partial water volume.

Fig.2. The mean refrac-tive index of different plankton species, based on Table 2, as a function of the partial water vo-lume.

-59-X

w a z

n

1.40

(.J

<(

~ u..

w 1.37

~

1.36

n

0.7

PARTIAL

v.. 0.9

VOLUME

1.4 5 ~--+---+----1---+----1

.5

.6 .7 .8 .9 1.0

Fig.3. Dispersion of the main algal constituents, relative to water.

Fig.4. Absorption in-dex K and refractive index n of an ideali-zed homogeneous alga, due to chlorophyll a

Fig.5.

Absorp-tion and scattering efficiencies for

dif-ferent diameters, when the chlorophyll

a content is 10 mg cm- 3 .

Fig.6. Absorption and scattering effi-ciencies for

diffe-

-61-0.0 0 1 L...-...i-....J~__._..._._-L.._,_--<--J

600 700 nm

100 J.lffi

rent diameters, when 0.11--+---1-+-t--, the chlorophyll a

content is 1 mg

c~- 3.

600

10

0. 1

0.0 1

100 J.lm

- - X ' = X'(~) , n =nO)

· · ... · K = 'r<i( t.) , n = N

- "':' - -X= 0 ' n = N

1J.1m

600 700 nm

10 J.lffi 100 jJffi

---

1 - - lJJm

-

---600 700 nm

COMMENTS AND CORRECTIONS TO REPORT N0.46:

THE REFRACTIVE INDEX OF PHYTOPLANKTON

Chapter 4.1, page 22

The waterish type of opal, with p=2.07 g em -3 and n=l.43, was chosen for the calculations since the opal of the phytoplankton contains water. However, the relative mass concen-trations of s~lica given in Table 2 (page 18) probably represents dry Sio

2 without any water content. More correct values for the calculations are then perhaps p=2.65 and n=l.486.

This change leads to some corrections.

lines in Table 8 (page 29) should now be

Diatoms .1. 538. 1. 4 3 8. II 1.536 Mean from Table 2 1.555 1.438 II 1. 342

~ine no.9 on page 30 should read:

n

~ (1.55 ± 0.01) - (0.21 ± O.Ol)vw and line no.l5:

Two ot the

1.281 II 1.201 II

density becomes (1.10 ± 0.05)g em .:..3 If only species with

Fig.2 consists of six curves for n as a function of c .

w

The lowest of these curves, for .diatoms, should now be omitted, since the new curve for diatoms will coincide with the one

for blue-green algae.

In document The refractive index of phytoplankton (sider 44-63)