4. METHODOLOGY
4.2. Qualitative methodology
4.2.11. Reliability
Além de permitir uma análise da cinética da expressão do EMMPRIN durante o desenvolvimento da lesão, os resultados obtidos com este modelo experimental de indução de lesão periapical sugerem que o EMMPRIN, como um indutor de MMPs, pode estar relacionado ao desenvolvimento da lesão periapical.
No entanto, estudos adicionais são necessários para determinar o papel exato do EMMPRIN no desenvolvimento da lesão. Se for comprovada a relação entre EMMPRIM e lesão periapical, a análise dessa proteína no exudato periapical ou no fluido crevicular, poderia ser utilizada para auxiliar no diagnóstico e na seleção da técnica de tratamento.
*De acordo com o manual da FOAr/UNESP, adaptadas das normas Vancouver. Disponível no site: http://www.nlm.nih.gov/bsd/uniform_requirements.html
Referências*
1. Allan JA, Docherty AJ, Barker PJ, Huskisson NS, Reynolds JJ, Murphy G. Binding of gelatinases A and B to type-I collagen and other matrix components. Biochem J 1995; 309 ( Pt 1): 299-306.
2. Bauvois B. New facets of matrix metalloproteinases MMP-2 and MMP-9 as cell surface transducers: outside-in signaling and relationship to tumor progression. Biochim Biophys Acta. 2012; 1825 (1): 29-36.
3. Beklen A, Ainola M, Hukkanen M, Gurgan C, Sorsa T, Konttinen YT. MMPs, IL-1, and TNF are regulated by IL-17 in periodontitis. J Dent Res. 2007; 86 (4): 347-51. 4. Belmar MJ, Pabst C, Martinez B, Hernandez M. Gelatinolytic activity in gingival
crevicular fluid from teeth with periapical lesions. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008; 105 (6): 801-6.
5. Birkedal-Hansen H. Role of matrix metalloproteinases in human periodontal diseases. J Periodontol. 1993; 64 (5 Suppl): 474-84.
6. Birkedal-Hansen H. Role of cytokines and inflammatory mediators in tissue destruction. J Periodontal Res. 1993; 28: 500-10.
7. Birkedal-Hansen H, Moore WG, Bodden MK, Windsor LJ, Birkedal-Hansen B, DeCarlo A, et al. Matrix metalloproteinases: a review. Crit Rev Oral Biol Med. 1993; 4 (2): 197-250.
8. Biswas C, Zhang Y, DeCastro R, Guo H, Nakamura T, Kataoka H, et al. The human tumor cell-derived collagenase stimulatory factor (renamed EMMPRIN) is a member of the immunoglobulin superfamily. Cancer Res. 1995;55 (2): 434-9.
9. Braundmeier AG, Dayger CA, Mehotra P, Belton RJ, Jr., Nowak RA. EMMPRIN is secreted by human uterine epithelial cells in microvesicles and stimulates metalloproteinase production by human uterine fibroblast cells. Reprod Sci 2012; 19 (12): 1292-301.
10. Buzoglu HD, Unal H, Ulger C, Mert S, Kucukyildirim S, Er N. The zymographic evaluation of gelatinase (MMP-2 and -9) levels in acute and chronic periapical abscesses. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009; 108 (5): e 121-6. 11. Cao Z, Xiang J, Li C. A potential role of EMMPRIN by regulating ECM degradation in
periodontitis. Med Hypotheses. 2007; 69 (5): 1102-4.
12. Carneiro E, Menezes R, Garlet GP, Garcia RB, Bramante CM, Figueira R, et al. Expression analysis of matrix metalloproteinase-9 in epithelialized and nonepithelialized apical periodontitis lesions. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009; 107 (1): 127-32.
13. Caudroy S, Polette M, Nawrocki-Raby B, Cao J, Toole BP, Zucker S, et al. EMMPRIN- mediated MMP regulation in tumor and endothelial cells. Clin Exp Metastasis. 2002; 19 (8): 697-702.
14. Corotti MV, Zambuzzi WF, Paiva KB, Menezes R, Pinto LC, Lara VS, et al. Immunolocalization of matrix metalloproteinases-2 and -9 during apical periodontitis development. Arch Oral Biol. 2009; 54 (8): 764-71.
15. Coussens LM, Fingleton B, Matrisian LM. Matrix metalloproteinase inhibitors and cancer: trials and tribulations. Science. 2002; 295 (5564): 2387-92.
16. de Paula-Silva FW, D'Silva NJ, da Silva LA, Kapila YL. High matrix metalloproteinase activity is a hallmark of periapical granulomas. J Endod. 2009; 35 (9): 1234-42.
17. DeCastro R, Zhang Y, Guo H, Kataoka H, Gordon MK, Toole B, et al. Human keratinocytes express EMMPRIN, an extracellular matrix metalloproteinase inducer. J Invest Dermatol. 1996; 106 (6): 1260-5.
18. Delaissé JM, Eeckhout Y, Neff L, François-Gillet C, Henriet P, Su Y, Vaes G, Baron R. (Pro)collagenase (matrix metalloproteinase-1) is present in rodent osteoclasts and in the underlying bone-resorbing compartment. J Cell Sci. 1993;106 ( Pt 4): 1071-82.
19. Dezerega A, Madrid S, Mundi V, Valenzuela MA, Garrido M, Paredes R, et al. Pro- oxidant status and matrix metalloproteinases in apical lesions and gingival crevicular fluid as potential biomarkers for asymptomatic apical periodontitis and endodontic treatment response. J Inflamm. (Lond) 2012; 9 (1): 8.
20. Donadio AC, Remedi MM, Susperreguy S, Frede S, Gilardoni MB, Tang Y, et al. Extracellular matrix metalloproteinase inducer (EMMPRIN) and matrix metalloproteinases (MMPs) as regulators of tumor-host interaction in a spontaneous metastasis model in rats. Histochem Cell Biol. 2008; 130 (6): 1155-64.
21. Dong W, Xiang J, Li C, Cao Z, Huang Z. Increased expression of extracellular matrix metalloproteinase inducer is associated with matrix metalloproteinase-1 and -2 in gingival tissues from patients with periodontitis. J Periodontal Res. 2009; 44 (1): 125- 32.
22. Emingil G, Atilla G, Sorsa T, Tervahartiala T. The effect of adjunctive subantimicrobial dose doxycycline therapy on GCF EMMPRIN levels in chronic periodontitis. J Periodontol. 2008; 79 (3): 469-76.
23. Emingil G, Tervahartiala T, Mantyla P, Maatta M, Sorsa T, Atilla G. Gingival crevicular fluid matrix metalloproteinase (MMP)-7, extracellular MMP inducer, and tissue inhibitor of MMP-1 levels in periodontal disease. J Periodontol. 2006; 77 (12): 2040-50.
24. Feldman M, La VD, Lombardo Bedran TB, Palomari Spolidorio DM, Grenier D. Porphyromonas gingivalis-mediated shedding of extracellular matrix metalloproteinase inducer (EMMPRIN) by oral epithelial cells: a potential role in inflammatory periodontal disease. Microbes Infect. 2011; 13 (14-15): 1261-9.
25. Gabison EE, Hoang-Xuan T, Mauviel A, Menashi S. EMMPRIN/CD147, an MMP modulator in cancer, development and tissue repair. Biochimie. 2005; 87 (3-4): 361-8. 26. Grass GD, Bratoeva M, Toole BP. Regulation of invadopodia formation and activity by
CD147. J Cell Sci. 2012; 125 (Pt 3): 777-88.
27. Graves DT, Oates T, Garlet GP. Review of osteoimmunology and the host response in endodontic and periodontal lesions. J Oral Microbiol. 2011; 3: 1-15.
28. Guo H, Zucker S, Gordon MK, Toole BP, Biswas C. Stimulation of matrix metalloproteinase production by recombinant extracellular matrix metalloproteinase inducer from transfected Chinese hamster ovary cells. J Biol Chem. 1997; 272 (1): 24-7. 29. Gusman H, Santana RB, Zehnder M. Matrix metalloproteinase levels and gelatinolytic activity in clinically healthy and inflamed human dental pulps. Eur J Oral Sci. 2002; 110 (5): 353-7.
30. Jouneau S, Khorasani N, P DES, Macedo P, Zhu J, Bhavsar PK, et al. EMMPRIN (CD147) regulation of MMP-9 in bronchial epithelial cells in COPD. Respirology. 2011; 16 (4): 705-12.
31. Kanyenda LJ, Verdile G, Boulos S, Krishnaswamy S, Taddei K, Meloni BP, et al. The dynamics of CD147 in Alzheimer's disease development and pathology. J Alzheimers Dis. 2011; 26 (4): 593-605.
32. Kasinrerk W, Fiebiger E, Stefanova I, Baumruker T, Knapp W, Stockinger H. Human leukocyte activation antigen M6, a member of the Ig superfamily, is the species
homologue of rat OX-47, mouse basigin, and chicken HT7 molecule. J Immunol. 1992; 149 (3): 847-54.
33. Kataoka H, DeCastro R, Zucker S, Biswas C. Tumor cell-derived collagenase- stimulatory factor increases expression of interstitial collagenase, stromelysin, and 72- kDa gelatinase. Cancer Res. 1993; 53 (13): 3154-8.
34. Konttinen YT, Li TF, Mandelin J, Liljestrom M, Sorsa T, Santavirta S, et al. Increased expression of extracellular matrix metalloproteinase inducer in rheumatoid synovium. Arthritis Rheum 2000; 43 (2): 275-80.
35. Lin SK, Chiang CP, Hong CY, Lin CP, Lan WH, Hsieh CC, Kuo MY. Immunolocalization of interstitial collagenase (MMP-1) and tissue inhibitor of metalloproteinases-1 (TIMP-1) in radicular cysts. J Oral Pathol Med. 1997; 26 (10): 458-63.
36. Lin SK, Kok SH, Kuo MY, Wang TJ, Wang JT, Yeh FT, et al. Sequential expressions of MMP-1, TIMP-1, IL-6, and COX-2 genes in induced periapical lesions in rats. Eur J Oral Sci. 2002; 110 (3): 246-53.
37. Liu L, Li C, Cai C, Xiang J, Cao Z. Cyclophilin A (CypA) is associated with the inflammatory infiltration and alveolar bone destruction in an experimental periodontitis. Biochem Biophys Res Commun. 2010; 391 (1): 1000-6.
38. Liu L, Li C, Cai X, Xiang J, Cao Z, Dong W. The temporal expression and localization of extracellular matrix metalloproteinase inducer (EMMPRIN) during the development of periodontitis in an animal model. J Periodontal Res. 2010; 45 (4): 541-9.
39. Liu Y, Min D, Bolton T, Nube V, Twigg SM, Yue DK, et al. Increased matrix metalloproteinase-9 predicts poor wound healing in diabetic foot ulcers. Diabetes Care. 2009; 32 (1): 117-9.
40. Major TC, Liang L, Lu X, Rosebury W, Bocan TM. Extracellular matrix metalloproteinase inducer (EMMPRIN) is induced upon monocyte differentiation and is expressed in human atheroma. Arterioscler Thromb Vasc Biol. 2002; 22 (7): 1200-7. 41. Marmorstein AD, Gan YC, Bonilha VL, Finnemann SC, Csaky KG, Rodriguez-Boulan
E. Apical polarity of N-CAM and EMMPRIN in retinal pigment epithelium resulting from suppression of basolateral signal recognition. J Cell Biol. 1998; 142 (3): 697-710. 42. Martins VL, Caley M, O'Toole EA. Matrix metalloproteinases and epidermal wound
repair. Cell Tissue Res. 2013; 351 (2): 255-68.
43. Matsui H, Yamasaki M, Nakata K, Amano K, Nakamura H. Expression of MMP-8 and MMP-13 in the development of periradicular lesions. Int Endod J. 2011; 44 (8): 739-45. 44. Menezes-Silva R, Khaliq S, Deeley K, Letra A, Vieira AR. Genetic susceptibility to
periapical disease: conditional contribution of MMP2 and MMP3 genes to the development of periapical lesions and healing response. J Endod. 2012; 38 (5): 604-7. 45. Muramatsu T, Miyauchi T. Basigin (CD147): a multifunctional transmembrane protein
involved in reproduction, neural function, inflammation and tumor invasion. Histol Histopathol. 2003; 18 (3): 981-7.
46. Nabeshima K, Iwasaki H, Koga K, Hojo H, Suzumiya J, Kikuchi M. Emmprin (basigin/CD147): matrix metalloproteinase modulator and multifunctional cell recognition molecule that plays a critical role in cancer progression. Pathol Int. 2006; 56 (7): 359-67.
47. Nanci A, Bosshardt DD. Structure of periodontal tissues in health and disease. Periodontol 2000. 2006; 40: 11-28.
48. Noguchi Y, Sato T, Hirata M, Hara T, Ohama K, Ito A. Identification and characterization of extracellular matrix metalloproteinase inducer in human
endometrium during the menstrual cycle in vivo and in vitro. J Clin Endocrinol Metab. 2003; 88 (12): 6063-72.
49. Omi Y, Shibata N, Okamoto T, Obara T, Kobayashi M. The role of CD147 in the invasiveness of follicular thyroid carcinoma cells. Thyroid. 2012; 22 (4): 383-94.
50. Paula-Silva FW, da Silva LA, Kapila YL. Matrix metalloproteinase expression in teeth with apical periodontitis is differentially modulated by the modality of root canal treatment. J Endod. 2010; 36 (2): 231-7.
51. Ricucci D, Siqueira JF Jr. Biofilms and apical periodontitis: study of prevalence and association with clinical and histopathologic findings. J Endod. 2010; 36 (8): 1277-88. 52. Shapiro F. Bone development and its relation to fracture repair. the role of
mesenchymal osteoblasts and surface osteoblasts. Eur Cell Mater. 2008; 15 (1): 53-76. 53. Shin SJ, Lee JI, Baek SH, Lim SS. Tissue levels of matrix metalloproteinases in pulps
and periapical lesions. J Endod. 2002; 28 (4): 313-5.
54. Shin SJ, Lee W, Lee JI, Baek SH, Kum KY, Shon WJ, et al. Matrix metalloproteinase-8 and substance P levels in gingival crevicular fluid during endodontic treatment of painful, nonvital teeth. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2011; 112 (4): 548-54.
55. Stashenko P, Teles R, D'Souza R. Periapical inflammatory responses and their modulation. Crit Rev Oral Biol Med. 1998; 9 (4): 498-521.
56. Stashenko P, Wang CY, Tani-Ishii N, Yu SM. Pathogenesis of induced rat periapical lesions. Oral Surg Oral Med Oral Pathol. 1994; 78 (4): 494-502.
57. Tang Y, Kesavan P, Nakada MT, Yan L. Tumor-stroma interaction: positive feedback regulation of extracellular matrix metalloproteinase inducer (EMMPRIN) expression and matrix metalloproteinase-dependent generation of soluble EMMPRIN. Mol Cancer Res. 2004; 2 (2): 73-80.
58. Tsai CH, Chen YJ, Huang FM, Su YF, Chang YC. The upregulation of matrix metalloproteinase-9 in inflamed human dental pulps. J Endod. 2005; 31 (12): 860-2. 59. Tsuji M, Yamasaki M, Amano K, Matsui H, Morimoto T, Nakamura H. Histochemical
localization of neutral proteases released during development of rat periradicular lesion. Arch Oral Biol. 2009; 54 (12): 1128-35.
60. Visse R, Nagase H. Matrix metalloproteinases and tissue inhibitors of metalloproteinases: structure, function, and biochemistry. Circ Res. 2003; 92 (8): 827- 39.
61. Wahlgren J, Salo T, Teronen O, Luoto H, Sorsa T, Tjaderhane L. Matrix metalloproteinase-8 (MMP-8) in pulpal and periapical inflammation and periapical root- canal exudates. Int Endod J. 2002; 35 (11): 897-904.
62. Wang CH, Dai JY, Wang L, Jia JF, Zheng ZH, Ding J, Chen ZN, Zhu P. Expression of CD147 (EMMPRIN) on neutrophils in rheumatoid arthritis enhances chemotaxis, matrix metalloproteinase production and invasiveness of synoviocytes. J Cell Mol Med. 2011; 15 (4): 850-60.
63. Wang CH, Yao H, Chen LN, Jia JF, Wang L, Dai JY, et al. CD147 induces angiogenesis through a vascular endothelial growth factor and hypoxia-inducible transcription factor 1alpha-mediated pathway in rheumatoid arthritis. Arthritis Rheum 2012; 64 (6): 1818-27.
64. Woessner JF, Nagase H. Matrix metalloproteinases and TIMPs. Oxford, UK: Oxford University Press; 2000.
65. Yang X, Zhang P, Ma Q, Kong L, Li Y, Liu B, et al. EMMPRIN silencing inhibits proliferation and perineural invasion of human salivary adenoid cystic carcinoma cells in vitro and in vivo. Cancer Biol Ther. 2012; 13 (2): 85-91.
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