7 CONCLUSÃO
Os resultados do presente trabalho nos permitem concluir que a desnutrição afetou a proliferação celular, retardando o ciclo celular.
O tratamento com o palmitato de retinol, mas não o retinol, diminuiu a taxa proliferativa e a apoptose. Ambos os tratamentos com os retinoides retardaram o ciclo celular quando comparados ao controle desnutrido e estimularam a diferenciação celular possivelmente por mecanismos associados com as vias moleculares do ATF2, MAPK/ERK/JNK, IL-6, Hedgehog, PI3K/AKT e NF-κB.
8 PERSPECTIVAS
Embora muito já tenha sido investigado, ainda necessita-se de mais estudos para melhor elucidação da hipótese proposta.
Assim, experimentos com métodos de supressão gênica e utilização de bloqueios farmacológicos serão necessários a fim de investigar se os efeitos dos retinoides nas vias propostas devem-se a ativação dos receptores acoplados à proteínas G, ou dos receptores retinoides RAR/RXR.
Além disso, as vias também podem ser investigadas mais detalhadamente, através de suas inibições, com o intuito de verificar o real efeito de cada via nas células intestinais tratadas com retinoides.
Os experimentos de análise proteica dos genes analisados já foram iniciados. Entretanto por motivos de problemas metodológicos esses experimentos foram temporariamente adiados, sendo necessário sua retomada.
Contudo os dados apresentados no presente estudo direcionam e norteiam as futuras pesquisas na identificação do mecanismo de ação modulatório dos retinoides nas células intestinais.
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