5 Diskusjon
5.2 Prinsipalkomponentanalyser
Em conclusão, os resultados do presente estudo ampliam a nossa compreensão sobre a regulação da expressão dos microRNAs na musculatura esqueletica humana em resposta ao treinamento físico aeróbico.
9. LIMITAÇÕES
Deve-se reconheçer uma série de limitações nesse estudo. A principal seria a relação estabelecia entre a expressão dos myomiRs, em especial do miR-206 e as expressões gênicas e proteicas encontradas. O estudo careceu de experimentos que traçassem uma correlação direta entre aumentos ou diminuição na expressão dos miRs e sua ação efetiva na musculatura esquelética (ensaio com luciferase). Assim, trabalhos da literatura já confirmaram em estudos in vitro e in vivo, essas alterações (McCarthy 2008). Entretanto, ações mecanicista em seres humanos carecem de mais estudos na literatura. Dessa forma, nosso trabalho tenta fazer uma contribuição nesse caminho. Outro ponto importante, foi que foram estudados indivíduos jovens e saudáveis, o que limita a extrapolação dos nossos resultados para outras faixas etárias e possíveis doenças, como distrofias. O mesmo argumento pode ser utilizado para a variável sexo. A amostra era constituída de apenas homens. Portanto, não se conhece os efeitos do treinamento físico aeróbio nos padrões de expressão dos myomiRs e da miogênese, em mulheres. Esses padrões de expressão poderiam ser alterados pelo ciclo hormonal que as mulheres são submetidas todos os meses.
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ANEXO I – Idade, altura, peso e índice de massa corpórea no pré e pós- treinamento físico aeróbio.
Pré Ano atual Nome Idade Altura-cm Peso IMC
GM-312 2011 D D C 25 168 75.8 26.8 GM-313 2011 L R M 27 172 76 25.6 GM-307 2011 R A O 26 175 86.7 28.3 GM-311 2011 W A S 29 186 109.7 31.7 GM-306 2011 I A B 29 177 102.7 32.7 GM-310 2011 F Q A 21 171 61.9 21.1 GM-314 2011 L F C 21 175 59.7 19.4 GM-308 2011 R G G 24 195 108.1 28.4 GM-315 2011 R M O 20 181 66.4 20.2 GM-316 2011 T F M G 22 171 74.8 25.5 GM-317 2011 W O S 26 171 69.6 23.8 GM-309 2011 A M S J 28 165 80.4 29.5
Pós Ano atual Nome Idade Altura-cm Peso IMC
GM-312 2012 D D C 25 169 72.4 25.35 GM-313 2012 L R M 28 172 74.2 25.08 GM-307 2012 R A O 26 175 84 27.43 GM-311 2012 W A S 30 186 103.7 29.97 GM-306 2012 I A B 29 176 100.4 32.41 GM-310 2012 F Q A 22 172 62.8 21.23 GM-314 2012 L F C 22 175 61.7 20.15 GM-308 2012 R G G 25 194 101.9 27.08 GM-315 2012 R M O 20 182 66.9 20.20 GM-316 2012 T F M G 23 171 69.6 23.80 GM-317 2012 W O S 26 172 67 22.65 GM-309 2012 A M S J 29 165 76.6 22.66
ANEXO II – Pressão arterial – Sistólica e Diastólica, no pré e pós-treinamento físico aeróbio.
PAS repouso
em pé PAD repouso PAM repouso PAS Pico PAD Pico PAM Pico
125 90 102 200 85 123 125 85 98 190 85 120 115 70 85 175 70 105 130 90 103 200 90 126 135 100 112 190 90 123 115 70 85 185 75 111 120 90 100 170 90 116 150 80 103 210 80 123 100 70 80 190 90 123 140 90 107 185 85 118 120 70 87 180 80 113 110 70 83 180 80 113 PAS repouso
em pé PAD repouso PAM repouso PAS Pico PAD Pico PAM Pico
130 100 110 215 100 138 130 70 90 170 70 103 120 80 93 195 85 121 130 100 110 240 120 160 135 90 105 210 80 123 120 90 100 200 80 120 110 80 90 180 80 113 120 80 93 190 80 116 130 100 110 180 90 120 120 80 93 185 80 115 130 80 110 195 80 118 120 80 93 185 80 115
ANEXO III – Consumo máximo de oxigênio – protocolo, tempos nos limiares – pré e pós-treinamento físico aeróbio.
Data do teste-
Vmax No do teste Protocolo
Tempo LA (seg) Tempo PCR (seg) tempo total exerc (seg) 18/08/2011 136Q Veloc-1 300 480 523 19/08/2011 133Q Veloc-1 300 498 600 16/09/2011 251Q Veloc-1 360 420 610 18/08/2011 134Q Veloc-1 300 390 605 15/08/2011 112Q Veloc-1 120 330 603 18/08/2011 135Q Veloc-1 210 390 566 22/08/2011 152Q Veloc-1 300 540 608 15/08/2011 113Q Veloc-1 300 420 480 22/08/2011 151Q Veloc-1 300 510 587 22/08/2011 149Q Veloc-1 300 480 601 22/08/2011 150Q Veloc-1 300 420 558 15/08/2011 114Q Veloc-1 360 430 485 Data do teste-
Vmax No do teste Protocolo
Tempo LA (seg) Tempo PCR (seg) tempo total exerc (seg) 29/02/2012 900Q Veloc-1 360 540 564 24/02/2012 874Q Veloc-1 378 618 543 24/02/2012 878Q Veloc-1 420 510 654 29/02/2012 896Q Veloc-1 330 420 600 24/02/2012 876Q Veloc-1 150 360 600 24/02/2012 875Q Veloc-1 330 540 632 29/02/2012 901Q Veloc-1 330 540 639 27/02/2012 886Q Veloc-1 300 420 500 29/02/2012 897Q Veloc-1 300 540 636 27/02/2012 885Q Veloc-1 450 600 660 28/02/2012 888Q Veloc-1 450 600 680 28/02/2012 889Q Veloc-2 400 550 670
ANEXO IV – Frequências cardíacas – nos limiares e percentuais, no pré e pós- treinamento físico aeróbio.
FC repouso- Em pé FC LA LA %Fcmax FC PCR PCR %Fcmáx FC máx 64 152 78 195 100 195 77 151 78 187 97 193 80 159 90 171 90 176 73 148 86 162 94 172 100 135 73 164 89 185 73 120 63 167 88 190 72 157 79 193 97 200 93 151 83 174 96 181 80 125 68 180 99 183 83 157 81 185 95 195 74 160 86 175 93 187 64 165 92 178 98 180 FC repouso- Em pé FC LA LA %Fcmax FC PCR PCR %Fcmáx FC máx 60 168 84 197 99 198 68 152 81 183 98 187 84 148 80 171 91 185 51 127 83 185 97 153 56 142 86 166 92 180 60 139 76 178 97 200 70 166 83 191 95 200 74 138 79 168 96 175 60 135 72 181 97 187 52 152 80 187 98 191 55 160 78 190 97 189 63 159 79 186 98 192
ANEXO V – Frequências cardíacas e consumo máximo de oxigênio (VO2máx.) -
nos limiares e percentuais, no pré e pós-treinamento físico aeróbio.
FCrec1 Delta MAX-1 % Fcmax pred (220-idade) % Fcmax pred (208- (0.7*idade) VO2baseline (ml/kg/min) VO2 LA (ml/kg/min) 171 24 195 191 3.7 30.1 163 30 193 189 3.5 30.9 153 23 194 190 3.5 35.3 145 27 191 188 3.6 30.9 160 25 191 188 3.5 28.3 159 31 199 193 3.5 22.4 173 27 199 193 3.9 33.2 152 29 196 191 3.2 27.5 156 27 200 194 4.3 30 160 35 198 193 5.7 42.2 153 34 194 190 3.0 32.9 150 30 192 188 4.0 37.8
FCrec1 Delta MAX-1 % Fcmax pred (220-idade) % Fcmax pred (208- (0.7*idade) VO2baseline (ml/kg/min) VO2 LA (ml/kg/min) 162 36 195 263 4.8 41.3 122 65 192 260 3.7 40.2 159 26 194 262 3.2 34.2 110 43 190 259 3.4 28.3 132 48 191 260 3.2 20.7 180 20 198 265 3.5 38.1 168 32 198 265 3.8 41.6 131 44 195 263 3.3 26.4 153 34 200 266 4.6 29.9 147 44 197 264 3.4 31,3 159 30 194 262 3.2 34.2 110 82 191 260 3.4 28.3
ANEXO VI – Consumo máximo de oxigênio (VO2máx.) - nos limiares e
percentuais, no pré e pós-treinamento físico aeróbio.
%VO2max LA VO2 PCR %VO2max PCR VO2max relativo (ml/kg/min) %VO2max ajustado idade pred 65 44.1 96 46 104 65 44.6 93 47.8 111 90 40.2 97 43.6 100 71 42.1 96 43.6 104 70 34.9 86 40.7 97 47 36 76 47.4 102 55 55.6 93 35.7 130 67 37.8 92 41 92 49 56.1 92 49.3 129 59 61.9 87 50 131 69 47.3 99 47.6 109 84 41 99 45.2 102
%VO2max LA VO2 PCR %VO2max PCR VO2max relativo (ml/kg/min) %VO2max ajustado idade pred 73 55.6 98 56.4 128 80 49.6 99 50.2 118 80 43.6 92 48 110 83 45.6 94 49.3 119 59 34.8 100 62.4 122 67 53.9 94 57.1 125 68 55.8 91 61.4 134 67 37.8 79 59.3 140 51 58.7 100 68.5 125 59 47 89 53 117 59 34.8 100 62.4 122 67 53.9 94 57.1 125
ANEXO VII – Consumo máximo de oxigênio (VO2máx.) - absolutos e RQ, no
pré e pós-treinamento físico aeróbio.
VO2max absoluto (L/min) VEbaseline (l/min) (BTPS) VE max (l/min) (BTPS) RQ rep RQ max 3.485 8.6 99.1 0.85 1.25 3.635 10.5 117.4 0.87 1.16 3.783 12 105.5 0.82 1.18 4.786 12.9 141.3 0.83 1.07 4.177 12 165.8 0.86 1.20 2.933 7.4 110.5 0.89 1.34 3.588 10.9 120.5 1.01 1.29 4.430 13 163.1 0.64 1.13 4.136 12.7 114.1 1.04 1.11 2.349 13.2 159.3 0.85 1.16 3.312 8.7 113.1 0.98 1.19 3.632 12.1 100.7 0.91 1.09 VO2max absoluto (L/min) VEbaseline (l/min) (BTPS) VE max (l/min) (BTPS) RQ rep RQ max 4.085 11.7 108.8 0.86 1.04 3.726 12.4 126 0.87 1.20 4.029 8.7 143.9 0.78 1.30 5.108 13.5 128.9 0.89 0.97 5.501 12.3 142 0.94 1.21 3.609 7.9 111.1 0.77 1.17 3.789 8.7 120.9 0.88 1.10 4.011 13.6 146.7 0.74 1.17 3.927 10 114.3 0.67 1.06 4.688 7.0 95.3 0.91 1.25 5.110 10.5 128.9 0.89 0.97 5.501 12.3 142 0.94 1.21
ANEXO VIII – Laudo ECG e velocidades atingidas na esteira – Cargas e percentuais, no pré e pós-treinamento físico aeróbio.
Laudo ECG Vel. Max
atingida (mph) Incli. Max atingida % Carga max Velocidade (km/h) Carga max inclinação (%) normal 7,8 9 12,5502 9 normal 8,2 11 13,1938 11 normal 7,8 9 12,5502 9 normal 7,8 9 12,5502 9 normal 7,8 9 12,5502 9 normal 8,2 11 13,1938 11 normal 8,5 13 13,6765 13 normal 7,8 9 12,5502 9 normal 8,2 11 13,1938 11 normal 8,5 13 13,6765 13 normal 8,2 11 13,1938 11 normal 7,8 9 12,5502 9
Laudo ECG Vel. Max
atingida Incli. Max atingida % Carga max Velocidade (km/h) Carga max inclinação (%) normal 8.2 11 13,1938 11 normal 8.6 17 13,8374 17 normal 8.5 13 13,6765 13 normal 7,4 11 11,9066 7 normal 7.4 9 11,9066 7 normal 8.5 13 13,6765 13 normal 8.5 13 13,6765 13 normal 7.8 11 12,5502 9 normal 8,5 13 13,6765 13 normal 8.5 13 13,6765 13 normal 7,1 11 11,4239 7 normal 7.4 11 11,9066 7