Sequence 1
Total ORFs Hits No hits
MEGABLAST 79 36 43
BLASTn 79 79 0
Hits on MEGABLAST
ORF name
Length (bp)
Organism Accession Pairwise identity
EU140752.1 100 100 WP_072756907:
hypothetical protein
771 Planctomycetes bacterium
990 Planctomycetes bacterium
CP036525 82.0 93.03 0 TWU40200: putative nucleotidyltransferase S1 -
ORF0010
414 Roseimaritima ulvae
609 Rhodopirellula baltica
S1 - ORF0021
354 Planctomycetes bacterium
384 Planctomycetes bacterium
1116 Planctomycetes sp.
CP011270 69.6 87.63 3.88
× 10 -101
WP_068259366:
slipin family protein
S1 - ORF0038
1038 Planctomycetes bacterium
477 Methylocystis sp.
396 Planctomycetes bacterium
396 Rhodopirellula baltica
270 Rhodopirellula baltica
108 Rhodopirellula baltica
357 Rhodopirellula baltica
726 Planctomycetes bacterium
S1 - ORF0090
636 Rhodopirellula baltica
BX294153 77.3 97.33 2.99
× 10 -131
WP_068264266: HD domain-containing protein
S1 - ORF0094
279 Rhodopirellula baltica
792 Planctomycetes bacterium
321 Planctomycetes bacterium
552 Planctomycetes bacterium
CP036262 100 100 0 WP_146459133:
CIA30 family protein S1 -
ORF00114
123 Planctomycetes bacterium
CP036262 100 100 0 WP_007332630:
CoA-binding protein S1 -
ORF00122
390 Planctomycetes bacterium
CP036262 100 100 0 WP_068264250:
CoA-binding protein S1 -
ORF00123
267 Planctomycetes bacterium
735 Rhodopirellula baltica
867 Rhodopirellula baltica
S1 - ORF00147
513 pCC2FOS fosmid vector
EU140752.1 100 100 WP_171831280:
tyrosine-type
EU140752.1 100 100 WP_001302176:
hypothetical protein
EU140752.1 100 100 EEU3264372:
replication initiation
EU140752.1 100 100 WP_001365321:
hypothetical protein S1 -
ORF00155
1176 pCC2FOS fosmid vector
EU140752.1 100 100 WP_001304218:
plasmid-partitioning
EU140752.1 100 100 WP_059242500:
ParB/RepB/Spo0J family plasmid partition protein
BLASTn search of «No hits» on MEGABLAST
ORF name Length (bp) Identical proteins Grade (%) Number of
results (out of 10)
S1 - ORF0013 99 WP_162273174:
hypothetical protein [Rubripirellula obstinata]
73.5 10
S1 - ORF0014 495 WP_146516087:
rhodanese-like domain-containing protein [Rubripirellula amarantea]
92.7 10
S1 - ORF0016 102 -
S1 - ORF0017 96 -
S1 - ORF0018 99 -
S1 - ORF0023 951 QDU26629: Group II
intron-encoded protein LtrA [Planctomycetes bacterium ETA_A8]
86.7 10
S1 - ORF0024 471 REJ87213: group II intron
reverse
transcriptase/maturase [Planctomycetes bacterium]
88.5 10
S1 - ORF0025 105 -
S1 - ORF0026 96 -
S1 - ORF0034 651 WP_068258815: RNA
2'-phosphotransferase [Rubripirellula obstinata]
87.5 10
S1 - ORF0037 660 WP_146405649:
tyrosine-protein phosphatase [Planctomycetes bacterium Poly21]
95.4 10
S1 - ORF0041 264 WP_146462253: M28
S1 - ORF0046 225 TWT97173: hypothetical
protein Pla100_23220
S1 - ORF0060 1548 MBA3583771: transposase
[Gemmatimonadetes RelE/ParE family toxin [Planctomycetes bacterium EC9]
74.7 10
S1 - ORF0066 141 TWU39980: hypothetical
protein Q31b_32960 [Planctomycetes bacterium Q31b]
46.3 2
S1 - ORF0068 222 -
S1 - ORF0096 162 TWT51002: hypothetical
protein Pla22_37780
[Planctomycetes bacterium Pla52n]
S1 - ORF00129 474 WP_068264232:
hypothetical protein [Rubripirellula obstinata]
83.8 10
S1 - ORF00130 105 RLS74515: ISAs1 family
transposase, partial [Planctomycetes bacterium]
57.4 6
S1 - ORF00133 666 OYV96015: hypothetical
protein B7Z68_06230 [Acidobacteria bacterium 21-70-11]
45.0 10
S1 - ORF00135 117 -
S1 - ORF00136 456 AJY36415: putative lipo domain protein
[Burkholderia mallei]
26.6 4
S1 - ORF00138 1413 WP_008674207: HAMP
domain-containing histidine kinase
[Rhodopirellula sallentina]
88.9 10
S1 - ORF00142 429 WP_009095090:
aminoacyl-tRNA hydrolase [Rhodopirellula sp. SWK7]
92.3 10
No hits on MEGABLAST or BLASTn: 0
Sequence 2
Total ORFs Hits No hits
MEGABLAST 41 34 7
BLAStn 41 41 0
Hits on MEGABLAST
ORF name Length (bp)
Organism Accession Pairwise identity
100 100 EEV9030702hypothetical
protein:
100 100 ADE34479: hypothetical
protein
CP018760 86.9 100 0 WP_073245624:
tRNA-
S2 - ORF0010
2068 Maribacte r sp.
CP011318 84.4 100 0 WP_133689069.1:
polyphosphate kinase 1 S2 -
bifunctional pyr operon transcriptional DNA-binding LytR/AlgR family response regulator
LT629754 99.60 80.9 0 WP_133689060.1
galactose
LT629754 93.6 100 0 WP_073245651.1:30S
ribosomal protein S1 S2 -
S2 - ORF0027
2457 Maribacte r sp.
LT629754 84.7 100 0 TDT37164.1:
ATP-dependent Lon protease S2 - factor, ECF subfamily S2 -
family MFS transporter S2 -
glycoside hydrolase family 31 protein
S2 -
BLASTn search of «No hits» on MEGABLAST
ORF name Length (bp) Identical proteins Grade (%) Number of
results (out of 10)
S2 - ORF007 852 WP_073245622: EboA
domain-containing protein [Maribacter aquivivus]
94.7 10
S2 - ORF0011 246 WP_133689068: histidine
phosphatase family protein [Maribacter spongiicola]
96.9 10
S2 - ORF0014 333 WP_133689066:
ribonuclease Z [Maribacter spongiicola]
98.6 10
S2 - ORF0019 342 WP_133689061: DUF4907
domain-containing protein [Maribacter spongiicola]
93.4 10
S2 - ORF0022 1251 TDT37168: long-subunit
fatty acid transport protein [Maribacter spongiicola]
97.8 10
S2 - ORF0028 120 -
S2 - ORF0033 735 WP_036158526: DUF2807
domain-containing protein [Maribacter forsetii]
93.6 10
No hits on Megablast or Blastn: 0
Sequence 3
Total ORFs Hits No hits
MEGABLAST 56 19 37
BLASTn 56 45 11
Hits on MEGABLAST
ORF name Length (bp)
Organism Accession Pairwise identity (%)
Query coverage (%)
E-value Protein
S3 -
S3 -
elongation factor 4 S3 -
ORF0037
894 Planctomycet es bacterium
CP036432 68.8 55.82****
*
1270 Crateriforma conspicua
acyl carrier protein S3 -
S3 -
BLASTn search of «No hits» on MEGABLAST
ORF name Length (bp) Identical proteins Grade
(%)
S3 - ORF0018 615 WP_052031530: class I SAM-dependent methyltransferase [Rhodopirellula maiorica]
79.8 10
S3 - ORF0020 618 EMI42599: putative membrane protein [Rhodopirellula sp. SWK7]
77.1 10
S3 - ORF0024 1086 WP_145098080: ROK family protein [Planctomycetes bacterium Poly24]
81.9 10
S3 - ORF0026 345 MAI72440: hypothetical protein [Rhodopirellula sp.]
65.3 10
S3 - ORF0027 102 -
S3 - ORF0029 2808 WP_154900441: circularly permuted type 2 ATP-grasp protein [Planctomycetes bacterium CA11]
70.8 10
S3 - ORF0030 885 WP_145262651: transglutaminase family protein [Planctomycetes bacterium Pan216]
76.6 10
S3 - ORF0031 792 WP_145419616: GIY-YIG nuclease family protein [Planctomycetes bacterium K23_9]
74.8 10
S3 - ORF0032 1101 QDV58998: hypothetical protein Mal33_50230 [Planctomycetes bacterium Mal33]
77.8 10
S3 - ORF0034 984 WP_164102765: D-2-hydroxyacid dehydrogenase [Roseimaritima sp. JC640]
85.0 10
S3 - ORF0035 1155 WP_164102793: protein kinase [Roseimaritima sp. JC640]
89.5 10
S3 - ORF0038 1227 WP_075084119:
endonuclease/exonuclease/phosphatase family protein [Mariniblastus fucicola]
74.6 10
S3 - ORF0039 456 WP_164101729: response regulator [Roseimaritima sp. JC640]
90.6 10
S3 - ORF0041 102 -
S3 - ORF0043 141 -
S3 - ORF0045 1428 WP_164104070: MFS transporter [Roseimaritima sp. JC640]
78.5 10
S3 - ORF0046 114 - 10
S3 - ORF0047 216 PHR99503: IS5/IS1182 family transposase [Blastopirellula sp.]
52.9 10
S3 - ORF0048 129 -
S3 - ORF0051 840 WP_164104021: purine-nucleoside phosphorylase [Roseimaritima sp. JC640]
89.1 10
S3 - ORF0052 126 -
S3 - ORF0053 1458 WP_146406530: sulfatase-like
hydrolase/transferase [Planctomycetes bacterium Poly21]
83.6 10
S3 - ORF0060 921 WP_164103642: ACP S-malonyltransferase [Roseimaritima sp. JC640]
84.9 10
S3 - ORF0062 96 GDX91350: 50S ribosomal protein L32 [Planctomycetia bacterium]
44.2 2
S3 - ORF0063 108 WP_153556032: 50S ribosomal protein L32 [Roseimaritima sp. JC651]
52.8 10
S3 - ORF0065 3069 WP_165225646: protein kinase [Aquisphaera sp.
JC669]
71.5 10
S3 - ORF0066 96 -
S3 - ORF0068 1203 WP_008662881: hypothetical protein [Rhodopirellula europaea]
74.1 10
S3 - ORF0069 1233 WP_145282472: PQQ-binding-like beta-propeller repeat protein [Planctomycetes bacterium Mal33]
75.9 10
S3 - ORF0072 102 -
S3 - ORF0073 1635 WP_145350034: von Willebrand factor type A domain-containing protein [Planctomycetes bacterium FF011L]
76.5 10
S3 - ORF0075 138 -
S3 - ORF0076 462 WP_145342310: low molecular weight phosphotyrosine protein phosphatase [Planctomycetes bacterium EC9]
78.5 10
S3 - ORF0078 276 WP_146592785: hypothetical protein [Planctomycetes bacterium Pla52o]
39.1 7
S3 - ORF0083 1173 WP_008693703: type II and III secretion system protein [Rhodopirellula maiorica]
74.2 10
No hits on MEGABLAST or BLASTn
-S3 - ORF0015 - S3 - ORF0016 - S3 - ORF0027 - S3 - ORF0041 - S3 - ORF0043 - S3 - ORF0046 - S3 - ORF0048
- S3 - ORF0066
Hits on MEGABLAST
ORF name
Length (bp)
Organism Accession Pairwise
identity
972 pCC2FOS fosmid vector
EU140752.1 100 100
S4 - ORF002
1167 pCC2FOS fosmid vector
EU140752.1 100 100
S4 - ORF005
756 pCC2FOS fosmid vector
EU140752.1 100 100
S4 - ORF007
126 pCC2FOS fosmid vector
EU140752.1 100 100
S4 - ORF009
120 pCC2FOS fosmid vector
EU140752.1 100 100
S4 - ORF0010
513 pCC2FOS fosmid vector
EU140752.1 100 100
S4 - ORF0012
660 pCC2FOS fosmid vector
EU140752.1 100 100
S4 - ORF0032
1824 Thermoleptolyngbya sp.
699 Geitlerinema sp. CP003591 71.6 81.26
5.98e-71
681 Oscillatoria acuminata
1920 Thermoleptolyngbya sp.
807 Thermoleptolyngbya sp.
732 Leptolyngbya sp. AP017367 77.3 31.28*** 1.58e-40
258 pCC2FOS fosmid vector
EU140752.1 100 100
S4 - ORF0080
159 pCC2FOS fosmid vector
EU140752.1 100 100
BLASTn search of «No hits» on MEGABLAST
ORF name Length (bp) Identical proteins Grade (%) Number of
results (out of 10)
S4 - ORF0015 1080 NJN29057: aromatic ring-hydroxylating dioxygenase subunit alpha [Synechococcales cyanobacterium RM1_1_8]
92.0 10
S4 - ORF0016 1959 NJM57392: FAD-dependent
oxidoreductase [Synechococcales cyanobacterium RU_4_20]
88.0 10
S4 - ORF0018 114 -
S4 - ORF0019 1827 NJN32773: alpha/beta hydrolase [Synechococcales
cyanobacterium RM1_1_8]
75.4 10
S4 - ORF0020 99 -
S4 - ORF0021 168 -
S4 - ORF0022 1347 BAZ30658: putative nicotinate phosphoribosyltransferase [Cylindrospermum sp. NIES-4074]
83.3 10
S4 - ORF0026 645 TVQ16582:
nicotinate-nucleotide adenylyltransferase [Leptolyngbya sp.
DLM2.Bin15]
73.0 10
S4 - ORF0030 756 NJM57395: NUDIX hydrolase
[Synechococcales cyanobacterium RU_4_20]
88.0 10
S4 - ORF0033 99 -
S4 - ORF0038 285 NJN32796: DUF427
domain-containing protein [Synechococcales
cyanobacterium RM1_1_8]
88.8 10
S4 - ORF0039 825 NJN32797: phytoene/squalene synthase family protein [Synechococcales
cyanobacterium RM1_1_8]
94.9 10
S4 - ORF0040 168 -
S4 - ORF0041 102 -
S4 - ORF0042 942 WP_009768167: M48 family
metalloprotease [Oscillatoriales
S4 - ORF0044 426 WP_058030484: hypothetical protein [Pseudoalteromonas
S4 - ORF0048 399 WP_168570017: DUF2605
domain-containing protein [Oxynema sp. AP17]
70.9 10
S4 - ORF0049 333 NJN31526: DUF2973
domain-containing protein [Synechococcales
cyanobacterium RM1_1_8]
65.1 10
S4 - ORF0051 147 -
S4 - ORF0052 1302 NJN30652: response regulator [Synechococcales
cyanobacterium RM1_1_8]
72.6 10
S4 - ORF0053 414 -
S4 - ORF0055 1233 WP_068514664: FIST
C-terminal domain-containing protein [Leptolyngbya sp. O-77]
80.0 10
S4 - ORF0056 228 WP_066349091: Calvin cycle protein CP12 [Geminocystis sp.
NIES-3708]
84.9 10
S4 - ORF0058 603 WP_068789457: DUF3177
family protein [Phormidium willei]
78.9 10
S4 - ORF0061 237 NJM48050: hypothetical protein [Alkalinema sp. RU_4_3]
80.1 10
S4 - ORF0064 822 WP_068510336:
FAD-dependent oxidoreductase [Leptolyngbya sp. O-77]
81.1 10
S4 - ORF0067 108 -
S4 - ORF0069 426 WP_162398825: hypothetical protein [Nostoc sp. B(2019)]
72.5 10
S4 - ORF0072 156 -
S4 - ORF0073 537 NJL85273: porin family protein [Leptolyngbyaceae
cyanobacterium SM1_1_3]
68.0 10
S4 - ORF0074 1218 WP_146133632:
tetratricopeptide repeat protein, partial [filamentous
cyanobacterium Phorm 46]
60.2 10
S4 - ORF0075 99 -
S4 - ORF0076 1101 NJR68231: signal peptidase I [Synechococcales
cyanobacterium CRU_2_2]
71.3 10
S4 - ORF0078 975 NJN32371: nuclear transport factor 2 family protein [Synechococcales
cyanobacterium RM1_1_8]
70.4 10
No hits on Megablast or Blastn
- S4 - ORF0018 - S4 - ORF0020 - S4 - ORF0021 - S4 - ORF0033 - S4 - ORF0040 - S4 - ORF0041 - S4 - ORF0051 - S4 - ORF0053 - S4 - ORF0067 - S4 - ORF0072 - S4 - ORF0075
Sequence 5
Total ORFs Hits No hits
MEGABLAST 62 59 3
BLASTn 62 56 6
Hits on MEGABLAST
ORF name
Length (bp)
Organism Accession Pairwise identity
S5 -
recognition particle
S5 -
S5 -
repair protein RecN
S5 - ORF0032
141 Klebsiella sp. CP056483 100 100
3.79e-64
WP_160886650:
hypothetical protein
S5 - family protein
S5 - ORF0037
477 Klebsiella sp. CP056483 100 100 0 WP_112217068: type
II toxin-antitoxin system RatA family toxin
S5 -
1821 Serratia marcescens
AP021873 92.0 100 0 WP_163525672:
DUF4365 domain-containing protein
S5 - ORF0047
1446 Cronobacter malonaticus
CP013940 78.2 96.89 0 WP_163525673:
SIR2 family protein
S5 - ORF0048
501 Cronobacter malonaticus
1479 Salmonella enterica
CP053332 82.4 100 0 WP_163525675:
relaxase/mobilization nuclease domain-containing protein S5 -
ORF0050
372 Klebsiella grimontii
270 Raoultella ornithinolytica
CP038281 93.7 100
1.73e-110
WP_163525677:
hypothetical protein
S5 - ORF0052
153 Raoultella ornithinolytica
CP038281 96.7 100
1.47e-63
WP_169050475:
hypothetical protein
S5 - ORF0053
303 Raoultella ornithinolytica
CP038281 97.4 100
7.85e-141
WP_163525678:
hypothetical protein
S5 - ORF0056
276 Raoultella ornithinolytica
CP038281 100 100
4.45e-137
WP_063407905:
hypothetical protein S5 -
hypothetical protein S5 -
hypothetical protein S5 -
AlpA family phage regulatory protein S5 -
S5 - transposase A S5 -
ORF0087
513 Klebsiella sp. CP056483 100 100 0 QLO35724: IS3
family transposase S5 -
ORF0088
249 Klebsiella sp. CP056483 100 100
1.79e-122
VGA88629: integrase catalytic subunit S5 -
171 Escherichia coli
CP056263 100 72.51
8.25e-55
BLASTn search of «No hits» on MEGABLAST
ORF NAME LENGTH (BP) IDENTICAL
PROTEINS
GRADE (%) NUMBER OF
RESULTS (OUT OF 10)
S5 - ORF0046 99 -
S5 - ORF0062 105 -
S5 - ORF0069 102 EAA4525289:
hypothetical protein
47.7 2
No hits on Megablast or Blastn
- S5 - ORF0021 - S5 - ORF0039 - S5 - ORF0046 - S5 - ORF0062 - S5 - ORF0067 - S5 - ORF0068
Sequence 6
Total ORFs Hits No hits
MEGABLAST 57 11 46
BLASTn 57 40 17
Hits on MEGABLAST
ORF name Length (bp)
Organism Accession Pairwise identity
855 pCC2FOS fosmid vector
EU140752.1 100 100
S6 - ORF002
1167 pCC2FOS fosmid vector
EU140752.1 100 100
S6 - ORF005
756 pCC2FOS fosmid vector
EU140752.1 100 100
S6 - ORF006
126 pCC2FOS fosmid vector
EU140752.1 100 100
S6 - ORF007
183 pCC2FOS fosmid vector
EU140752.1 100 100
S6 - ORF008
513 pCC2FOS fosmid vector
EU140752.1 100 100
S6 - ORF009
660 pCC2FOS fosmid vector
EU140752.1 100 100
S6 - ORF0016
1491 Halomicronema hongdechloris
585 Geobacter anodireducens
CP014963 67.10 74.36
6.90e-25
WP_153293456:
zeta toxin family protein
102 pCC2FOS fosmid vector
EU140752.1 100 100
BLASTn search of «No hits» on MEGABLAST
ORF name Length (bp) Identical proteins Grade (%) Number of
results (out of 10)
S6 - ORF0012 528 -
S6 - ORF0013 174 AFY93165: hypothetical
protein Cha6605_2070 [Chamaesiphon minutus PCC 6605]
44.0 7
S6 - ORF0014 3393 NEQ48243: hypothetical
protein [Leptolyngbya sp.
SIOISBB]
68.6 10
S6 - ORF0018 612 -
S6 - ORF0019 120 NJR70312: hypothetical
protein [Synechococcales cyanobacterium CRU_2_2]
60.3 1
S6 - ORF0020 540 NJN32002: hypothetical
protein [Synechococcales cyanobacterium RM1_1_8]
55.2 10
S6 - ORF0021 261 NJR67456: hypothetical
protein [Synechococcales cyanobacterium CRU_2_2]
83.1 1
S6 - ORF0023 399 NJN31714: hypothetical
protein [Synechococcales cyanobacterium RM1_1_8]
80.7 1
S6 - ORF0024 105 -
S6 - ORF0025 690 NJN31713: hypothetical
protein [Synechococcales cyanobacterium RM1_1_8]
70.6 2
S6 - ORF0027 216 WP_008317590: hypothetical protein [Leptolyngbya sp. PCC 6406]
49.7 10
S6 - ORF0030 1125 NJN31710: DGQHR [Synechococcus sp. PCC 7336]
49.6 10
S6 - ORF0036 534 NJN32748: hypothetical
protein [Synechococcales cyanobacterium RM1_1_8]
69.2 10
S6 - ORF0037 762 NEQ28405: ISKra4 family
transposase [Microcoleus sp.
SIO2G3]
85.6 10
S6 - ORF0038 210 NJR71155: hypothetical
protein [Synechococcales cyanobacterium CRU_2_2]
51.5 10
S6 - ORF0040 405 NJN32748: hypothetical
protein [Synechococcales cyanobacterium RM1_1_8]
81.7 3
S6 - ORF0042 864 NJN32749: hypothetical
protein [Synechococcales cyanobacterium RM1_1_8]
75.4 10
S6 - ORF0043 552 NJN32750: hypothetical
protein [Synechococcales
S6 - ORF0049 213 RPI65896:
protein-L-isoaspartate
O-33.1 3
methyltransferase, partial [Ignavibacteriae bacterium]
S6 - ORF0052 375 NJM58486: hypothetical
protein [Synechococcales cyanobacterium RU_4_20]
34.0 1
S6 - ORF0054 1089 NJM58485:
relaxase/mobilization nuclease domain-containing protein [Synechococcales cyanobacterium RU_4_20]
73.2 10
S6 - ORF0058 1533 NJN30180: hypothetical protein [Synechococcales cyanobacterium RM1_1_8]
87.7 10
S6 - ORF0060 258 -
S6 - ORF0062 390 NJR70879: hypothetical
protein [Synechococcales cyanobacterium CRU_2_2]
50.8 2
S6 - ORF0063 603 NJR70880: hypothetical
protein [Synechococcales cyanobacterium CRU_2_2]
87.1 10
S6 - ORF0064 96 -
S6 - ORF0065 96 -
S6 - ORF0066 186 NJN30184: hypothetical
protein [Synechococcales cyanobacterium RM1_1_8]
63.4 2
S6 - ORF0068 255 NJN30185: hypothetical
protein [Synechococcales cyanobacterium RM1_1_8]
83.3 2
S6 - ORF0069 171 -
S6 - ORF0070 228 -
S6 - ORF0071 234 -
S6 - ORF0072 321 NJN30946: hypothetical protein [Synechococcales cyanobacterium RM1_1_8]
83.5 3
S6 - ORF0073 126 -
S6 - ORF0074 129 -
S6 - ORF0075 1803 NJM58305: hypothetical
protein [Synechococcales cyanobacterium RU_4_20]
87.3 10
S6 - ORF0077 204 -
S6 - ORF0079 99 -
S6 - ORF0081 192 WP_144969979:
4a-hydroxytetrahydrobiopterin dehydratase [Bremerella volcania]
86.5 10
S6 - ORF0082 168 -
S6 - ORF0083 273 WP_166276346:
acetyltransferase [Aphanocapsa montana]
70.7 10
No hits on MEGABLAST or BLASTn
- S6 - ORF0012 - S6 - ORF0018 - S6 - ORF0024 - S6 - ORF0045 - S6 - ORF0047 - S6 - ORF0048 - S6 - ORF0060
- S6 - ORF0064 - S6 - ORF0065 - S6 - ORF0069 - S6 - ORF0070 - S6 - ORF0071 - S6 - ORF0073 - S6 - ORF0074 - S6 - ORF0077 - S6 - ORF0079 - S6 - ORF0082
Sequence 7 – contigs 1, 2, and 3
Total ORFs Hits No hits
MEGABLAST 44 23 21
BLASTn 44 39 5
Hits on MEGABLAST
ORF name
Length (bp)
Organism Accession Pairwise
identity
1023 Planctomycetes bacterium
1758 Rhodopirellula baltica
1089 Planctomycetes bacterium
261 pCC2FOS fosmid vector
EU140752.1 100 100
CONTIG 2 S7 - ORF009
120 pCC2FOS fosmid vector
EU140752.1 100 100
CONTIG 2 S7 - ORF0010
99 pCC2FOS fosmid vector
EU140752.1 100 100
CONTIG 2 S7 - ORF0011
102 pCC2FOS fosmid vector
EU140752.1 100 100
CONTIG 2 S7 - ORF0012
975 pCC2FOS fosmid vector
EU140752.1 100 100
CONTIG 2 S7 - ORF0014
1161 pCC2FOS fosmid vector
EU140752.1 100 100
CONTIG 2 S7 - ORF0015
96 pCC2FOS fosmid vector
EU140752.1 100 100
CONTIG 2 S7 - ORF0016
93 pCC2FOS fosmid vector
EU140752.1 100 100
CONTIG 2 S7 - ORF0017
126 pCC2FOS fosmid vector
EU140752.1 100 100
CONTIG 2 S7 - ORF0019
756 pCC2FOS fosmid vector
EU140752.1 100 100
CONTIG 2 S7 - ORF0020
147 pCC2FOS fosmid vector
EU140752.1 100 100
CONTIG 2 S7 - ORF0021
117 pCC2FOS fosmid vector
EU140752.1 100 100
CONTIG 2 S7 - ORF0022
330 pCC2FOS fosmid vector
EU140752.1 100 100
CONTIG 2 S7 - ORF0023
660 pCC2FOS fosmid vector
EU140752.1 100 100
CONTIG 2 S7 - ORF0028
1704 Rhodopirellula baltica
1323 Planctomycetes bacterium
WP_167546599 47.1 88.89 1.61e-110
558 Rhodopirellula europaea
WP_037251127 84.9 99.46 4.94e-112
666 Planctomycetaceae bacterium
5388 Verrucomicrobia bacterium
PYI87545 43.5 81.85 0 hypothetical protein DME26_05830, partial
BLASTn search of «No hits» on MEGABLAST
ORF name Length (bp) Identical proteins Grade (%) Number of
results (out of 10)
S7 - ORF001 660 WP_150074029: mucoidy
inhibitor MuiA family protein [Rhodopirellula sp.
JC645]
73.2 10
S7 - ORF002 102 -
S7 - ORF003 975 EMI22299: secreted protein
[Rhodopirellula maiorica SM1]
68.8 10
S7 - ORF005 2433 TWU45271: Periplasmic
beta-glucosidase precursor [Planctomycetes bacterium Q31b]
91.7 10
S7 - ORF007 1497 WP_009100429: TRAP
transporter large permease [Rhodopirellula sp. SWK7]
90.2 10
S7 - ORF009 462 WP_008673152: TRAP
transporter small permease [Rhodopirellula sallentina]
83.3 10
S7 - ORF0011 1071 WP_009100433: TRAP
transporter substrate-binding protein [Rhodopirellula sp.
SWK7]
[Planctomycetes bacterium Poly21]
S7 - ORF0023 1047 WP_146407289: biotin
synthase BioB
S7 - ORF0030 537 TWT74243: bifunctional
nicotinamide
S7 - ORF0032 1152 EMI42754: hypothetical
protein RRSWK_04943 [Rhodopirellula sp. SWK7]
79.4 10
1716 WP_081796966: tandem-95
repeat protein [Bacillus ndiopicus]
78.7 10
CONTIG 2 S7 - ORF0031
930 WP_146392717:
hypothetical protein [Rhodopirellula solitaria]
79.2 10
CONTIG 2 S7 - ORF0034
402 WP_146579500:
hypothetical protein
No hits on MEGABLAST or BLASTn
- S7 - ORF002 - S7 - ORF0015 - S7 - ORF0018 - S7 - ORF0033 - C3 S7- orf003
Sequence 8
Total ORFs Hits No hits
MEGABLAST 41 36 5
BLASTn 41 41 0
Hits on MEGABLAST
ORF name Length (bp)
Organism Accession Pairwise identity
2193 Sorangium cellulosum
2565 Rhodothermaceae bacterium
1419 Candidatus Snodgrassella
159 pCC2FOS fosmid vector
EU140752.1 100 100
S8 - ORF0011
99 pCC2FOS fosmid vector
EU140752.1 100 100
S8 - ORF0013
114 pCC2FOS fosmid vector
EU140752.1 100 100
S8 - ORF0014
114 pCC2FOS fosmid vector
EU140752.1 100 100
S8 - ORF0017
105 pCC2FOS fosmid vector
EU140752.1 100 100
S8 - ORF0019
126 pCC2FOS fosmid vector
EU140752.1 100 100
S8 - ORF0023
972 pCC2FOS fosmid vector
EU140752.1 100 100
S8 - ORF0028
1167 pCC2FOS fosmid vector
EU140752.1 100 100
S8 - ORF0029
102 pCC2FOS fosmid vector
EU140752.1 100 100
S8 - ORF0031
99 pCC2FOS fosmid vector
EU140752.1 100 100
S8 - ORF0032
129 pCC2FOS fosmid vector
EU140752.1 100 100
S8 - ORF0034
105 pCC2FOS fosmid vector
EU140752.1 100 100
S8 - ORF0035
93 pCC2FOS fosmid vector
EU140752.1 100 100
S8 - ORF0040
756 pCC2FOS fosmid vector
EU140752.1 100 100
S8 - ORF0041
96 pCC2FOS fosmid vector
EU140752.1 100 100
S8 - ORF0042
96 pCC2FOS fosmid vector
EU140752.1 100 100
S8 - ORF0043
108 pCC2FOS fosmid vector
EU140752.1 100 100
S8 - ORF0044
108 pCC2FOS fosmid vector
EU140752.1 100 100
S8 - ORF0047
120 pCC2FOS fosmid vector
EU140752.1 100 100
S8 - ORF0048
144 pCC2FOS fosmid vector
EU140752.1 100 100
S8 - ORF0052
513 pCC2FOS fosmid vector
EU140752.1 100 100
S8 - ORF0053
105 pCC2FOS fosmid vector
EU140752.1 100 100
S8 - ORF0054
105 pCC2FOS fosmid vector
EU140752.1 100 100
S8 - ORF0055
135 pCC2FOS fosmid vector
EU140752.1 100 100
S8 - ORF0056
195 pCC2FOS fosmid vector
EU140752.1 100 100
S8 - ORF0060
153 pCC2FOS fosmid vector beta-propeller fold lactonase family protein
S8 - ORF0068
639 Azotobacter chroococcum
1506 Pseudomonas oryzae
2895 Bradymonadales bacterium
1248 Gemmatirosa kalamazoonesis
2127 Gemmatirosa kalamazoonesis
CP007128 71.80 21.72 1.55e-52
HIG75890.1: sigma-70 family RNA polymerase sigma factor***
S8 - ORF0080
1623 Luteitalea pratensis
BLASTn search of «No hits» on MEGABLAST
ORF NAME LENGTH (BP) IDENTICAL
PROTEINS
GRADE (%) NUMBER OF
RESULTS (OUT OF 10)
S8 - ORF0064 1923 WP_143097359: S41
family peptidase [Myxococcus fulvus]
58.2 10
S8 - ORF0066 2973 WP_094548663:
TonB-dependent receptor [Rubricoccus marinus]
69.9 10
S8 - ORF0067 1455 NOS85174: RNA
polymerase sigma factor [Ignavibacteria
bacterium]
39.7 10
S8 - ORF0074 1587 WP_095514951:
serine/threonine protein kinase [Rubrivirga sp.
SAORIC476]
70.1 10
S8 - ORF0077 2310 MYF63479:
sulfatase-like
hydrolase/transferase [Rhodothermaceae bacterium]
43.3 10
No hits on MEGABLAST or BLASTn: 0
Sequence 9
Total ORFs Hits No hits
MEGABLAST 40 24 16
BLASTn 40 39 1
Hits on MEGABLAST
ORF name
Length (bp)
Organism Accession Pairwise identity
102 pCC2FOS fosmid vector
972 pCC2FOS fosmid vector
1176 pCC2FOS fosmid vector
756 pCC2FOS fosmid vector
96 pCC2FOS fosmid vector
126 pCC2FOS fosmid vector
120 pCC2FOS fosmid vector
513 pCC2FOS fosmid vector
660 pCC2FOS fosmid vector
EU140752.
1
100 100
S9 - ORF0017
2334 Granulosicoccus antarcticus
CP018632 68.30 45.59**
*
216 Roseobacter litoralis CP002623 80.60 99.54 6.4 6e-45
MPT24899.1:
acetamidase/formamida se family protein S9 - se family protein S9 - ATP-binding protein UrtD S9 -
ORF0031
1227 Marinobacter hydrocarbonoclastic permease subunit UrtC S9 -
ORF0032
927 Bradyrhizobium guangdongense permease subunit UrtB S9 -
ORF0035
1206 Confluentimicrobium sp.
CP010869 77.70 94.69 0 WP_153772203.1: urea ABC transporter substrate-binding protein
S9 - ORF0038
3435 Labrenzia sp. CP045380 67.20 37.73**
*
1803 Granulosicoccus antarcticus
CP018632 75.30 99.67 0 ASJ74785.1: putative oxidoreductase CzcO S9 -
ORF0049
549 Granulosicoccus antarcticus
1854 Granulosicoccus antarcticus
CP018632 74.80 97.09 0 NND91485.1:
excinuclease ABC subunit UvrC
S9 - ORF0052
972 Granulosicoccus antarcticus
1038 Granulosicoccus antarcticus
201 Granulosicoccus antarcticus
CP018632 75.80 96.52 1.1 4e-28
NND91481.1: 50S ribosomal protein L32
S9 -
terminase small subunit
BLASTn search of «No hits» on MEGABLAST
ORF name Length (bp) Identical proteins Grade (%) Number of
results (out of 10)
S9 - ORF0019 765 WP_088920696: sulfite
exporter TauE/SafE family protein [Granulosicoccus antarcticus]
80.4 10
S9 - ORF0020 597 WP_125926863:
MULTISPECIES: LEA type 2 family protein
[Pseudomonas]
65.5 10
S9 - ORF0023 186 WP_155846134: hypothetical
protein [Celeribacter ethanolicus]
46.8 10
S9 - ORF0024 1395 RZO33341: ammonium
transporter [SAR116 cluster bacterium]
86.1 10
S9 - ORF0025 1818 HIC47449: transporter substrate-binding
domain-72.4 10
containing protein [Methylophaga sp.]
S9 - ORF0026 225 WP_136385948: zinc ribbon
domain-containing protein [Azoarcus sp. CC-YHH848]
49.3 10
S9 - ORF0044 498 OED37980: hypothetical
protein AB833_21610 [Chromatiales bacterium (ex Bugula neritina AB1)]
78.3 10
S9 - ORF0045 153 -
S9 - ORF0046 144 WP_146366491: ABC
transporter permease [Litoreibacter sp. LN3S51]
47.7 10
S9 - ORF0047 282 WP_142905356:
helix-turn-helix domain-containing protein [Exilibacterium tricleocarpae]
87.1 10
S9 - ORF0048 1260 RLA50911: type II toxin-antitoxin system HipA family toxin [Gammaproteobacteria
transcription factor [Stenotrophobium rhamnosiphilum]
S9 - ORF0056 582 WP_157736169: DUF177
domain-containing protein [Granulosicoccus antarcticus]
76.6 10
No hits on MEGABLAST or BLASTn:
- S9 - ORF0045
Sequence 10
Total ORFs Hits No hits
MEGABLAST 56 24 32
BLASTn 56 46 10
Hits on MEGABLAST
ORF name Length (bp)
Organism Accession Pairwise identity (%)
Query coverage (%)
E-value
Protein
S10 - ORF001
972 pCC2FOS
fosmid vector
EU140752.1 100 100
S10 - ORF002
1158 pCC2FOS fosmid vector
EU140752.1 100 100
S10 - ORF003
102 pCC2FOS
fosmid vector
EU140752.1 100 100
S10 -
624 Uncultured bacterium
MG458673 93.7 17.63*** 3.58e-35
WP_007329889:
flagellar basal body P-ring formation protein FlgA S10 -
ORF0013
804 Rhodopirellula baltica
BX294154 77.5 99.75
1.42e-174
MAP08669:
flagellar basal-body rod protein FlgG [Rhodopirellula sp.]
S10 - ORF0026
2106 Rhodopirellula baltica
381 Rhodopirellula baltica
BX294151 81.0 86.88
5.25e-81
WP_009099718:
DUF3467 domain-containing protein S10 -
ORF0036
2238 Rhodopirellula baltica
BX294151 77.4 99.91 0 TWT56412:
putative peptide zinc metalloprotease protein YydH [Rhodopirellula solitaria]
S10 - ORF0040
2052 Rhodopirellula baltica
BX294151 73.8 98.98 0 WP_008679296:
HlyD family efflux transporter periplasmic adaptor subunit
S10 - ORF0047
1452 Rhodopirellula baltica
BX294144 74.1 99.04 0 WP_083904879:
RNA polymerase factor sigma-54 S10 -
ORF0054
1881 Rhodopirellula baltica
BX294144 77.1 58.37** 0 WP_146389752:
DNA polymerase III subunit gamma/tau S10 -
ORF0061
1032 Rhodopirellula baltica
3492 Rhodopirellula baltica
BX294143 74.5 72.37 0 EMI45813:
transcription-repair coupling factor S10 -
ORF0069
1041 Rhodopirellula baltica
1005 Rhodopirellula baltica
579 Rhodopirellula baltica
BX294141 75.3 71.50
5.59e-64
WP_008681921:
Flp family type IVb pilin
BLASTn search of «No hits» on MEGABLAST
ORF name Length (bp) Identical proteins Grade (%) Number of results (out of 10)
S10 - ORF0014 753 WP_009102573:
flagellar hook basal-body protein [Rhodopirellula sp.
SWK7]
92.6 10
S10 - ORF0017 465 WP_044256526:
hypothetical protein [Rhodopirellula sp.
SWK7]
92.5 10
S10 - ORF0019 96 -
S10 - ORF0020 159 -
S10 - ORF0021 120 -
S10 - ORF0023 522 TWT87892:
Inosine-5'-monophosphate dehydrogenase [Planctomycetes bacterium Pla100]
90.2 10
S10 - ORF0025 1701 WP_146407640:
hypothetical protein [Planctomycetes bacterium Poly21]
78.0 10
S10 - ORF0027 723 WP_044302358:
ATP-binding cassette domain-containing protein [Rhodopirellula sallentina]
93.1 10
S10 - ORF0028 93 -
S10 - ORF0031 93 -
S10 - ORF0032 729 EMI55677: protein
containing DUF556 [Rhodopirellula sallentina SM41]
72.0 10
S10 - ORF0038 945 WP_085981221: efflux
[Planctomycetes
pilus assembly protein CpaB [Rhodopirellula sp. SWK7]
86.3 10
S10 - ORF0094 1785 WP_009100158: pilus
assembly protein
S10 - ORF0097 561 WP_009100154:
MinD/ParA family protein [Rhodopirellula sp. SWK7]
90.2 10
No hits on MEGABLAST or BLASTn
- S10 - ORF0019 - S10 - ORF0020 - S10 - ORF0021 - S10 - ORF0028 - S10 - ORF0031 - S10 - ORF0041 - S10 - ORF0048 - S10 - ORF0075 - S10 - ORF0089 - S10 - ORF0096