O BE pode ser o causador de um estresse oxidativo que afetou a atividade das enzimas envolvidas na degradação de ROS. Pode-se observar também, a atividade da GR para a espécie de D. melanogaster, fato esse não descrito anteriormente na literatura. Além disso, a GR apresentou uma expressão diferenciada entre os grupos tratados e o grupo controle.
REFERÊNCIAS:
AYRES, M.; AYRES, M. Jr.; AYRES, D.L.; SANTOS, A.S. BioEst 4.0: Aplicações
Estatísticas nas Áreas das Ciências Biológicas e Médicas. Editora: Sociedade Civil
Mamirauá/ MCT/ Imprensa Oficial do Estado do Pará, 2005.
ATTIA, S.M.; SCHMID, T.E.; BADARY, A.O.; HAMADA, F.M.; ADLER, I.D. Molelular cytogenetic analysis in mouse sperm of chemically induced aneuploidy: studies with topoisomerase II inhibitors. Mutat Res, v. 520, pp. 1-13, 2002.
&DStWXOR,9&DStWXOR,9&DStWXOR,9&DStWXOR,9113
BEUTLER, E. Disorders in glutathione metabolism. Life Sci., v. 16(10), p.1499-505, 1975.
BIERKENS, J. G. E. A. Heat shock proteins in ecotoxicological risk assessent.
Advances in Molecular Toxicology, Utrecht, p. 531-544, 1998.
BIERKENS, J.G. Applications and pitfalls of stress-proteins in biomonitoring.
Toxicology., v.153(1-3), pp.61-72, 2000
BRADFORD, M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem., v. 72, p. 248-54, 1976.
CARLBERG, I.; MANNERVIK, B. Glutathione reductase. Methods Enzymol., v. 113, p.484-90, 1985.
COCHRANE, B.J.; RICHMOND, R.C. Studies of esterase-6 in Drosophila
melanogaster. II. The genetics and frequency distributions of naturally occurring
variants studied by electrophoretic and heat stability criteria. Genetics, v. 93(2), pp. 461-78, 1979.
CHOWDHURI, D. K.; SAXENA, D. K.; VISHWANATHAN, P. N. Effect of hexachlorocyclohexane (HCH), its isomers, and metabolites on Hsp70 expression in transgenic Drosophila melanogaster. Pestic. Biochem. Physiol., v.63, pp.15-25, 1999. DOUGLAS, K.T. Mechanism of action of glutathione-dependent enzymes. Adv.
Enzymol. Relat. Areas Mol. Biol., v. 59, p. 103–67, 1987.
FRÉMONT, L. Biological effects of resveratrol. Life Sci., v. 66, p. 663-73, 2000. GIRARD, P.; PALABOST, L.; PETITI, C. Enzymatic variation at seven loci in nine natural populations of Drosophila melanogaster. Biochem Genet. v.15(5-6), p. 589-99, 1977.
GODARD, T.; DESLANDES, E.; SICHEL, F.; POUL, J.M; GAUDUCHON, P. Detection of topoisomerase inhibitor-induced DNA strand breaks and apoptosis by alkaline comer assay. Cytol, v. 62, p. 39-45, 2002.
HALLIWELL, B. Antioxidant characterization, methodology and mechanism. Biochem.
Pharm., v.49, p. 1341-1348, 1995.
HEINEN E. Effects of antimitotic agents either free or bound to DNA on mouse peritoneal macrophages cultivated in vitro. Virchows Arch B Cell Pathol, v. 27, p. 79- 87, 1978.
KANZOK, S. M.; et al. Substitution of the Thioredoxin System for Glutathione Reductase in Drosophila melanogaster. Science, v. 291, p. 643-646, 2001
&DStWXOR,9&DStWXOR,9&DStWXOR,9&DStWXOR,9114
KEEN, J.H., HABIG, W.H., JAKOKI, W.B. Mechanism for the several activities of the glutathione S-transferases. J Biol Chem., v. 251(20), p.6183-8, 1976.
KIANG, J. G.; TSOKOS, G. C. Heat Shock Protein 70 kDa: Molecular Biology, Biochemistry, and Physiology. Pharmacology, v. 80, p. 183-201, 1998.
KREBS, R.A.; FEDER, M.E. Tissue-specific variation in HSP70 expression and thermal damage in Drosophila melanogaster larvae. J. Exp. Biol., v. 200, p. 2007-2015, 1997.
McCANN, J.; CHOI, E.; YAMASAKI, E.; AMES B.N. Detection of carcinogens as mutagens in the Salmonella/microsome test: assay of 300 chemicals. Proc. Natl. Acad.
Sci., v. 72, p.5135-9, 1975.
MATÉS, J. S.; SÁNCHEZ-JIMÉNEZ, F. Antioxidant enzymes and their implications in pathophysiologic processes. Front. Biosc, v. 4, 1999.
McCord, J.M. ; Fridovich, I. The utility of superoxide dismutase in studying free radical reactions. I. Radicals generated by the interaction of sulfite, dimethyl sulfoxide, and oxygen. J Biol Chem., v. 244(22), p.6056-63, 1969.
MEDEIROS, H.F.; KLACZKO, L.B. A weakly biased Drosophila trap. Dros. Inf. Serv., v. 82, p. 100-102.
MEISTER, A. Glutathione metabolism and its selective modification. J. Biol. Chem., v. 263 (33),p. 17205–8, 1988.
MENA, S.; ORTEGA, A.; ESTRELA, J. Oxidative stress in environmental-induced carcinogenesis. Mutation Research, v. 674, p. 36-44, 2009.
MO, Y.Y.; BECK, W.T. Association of human DNA topoisomerase II alpha with mitotic chromosomes in mammalian cells is independent of its catalytic activity. Exp
Cell Res., v. 252(1), pp. 50-62, 1999.
MUKHOPADHYAY, I.; NAZIR, A.; SAXENA, D.K.; CHOWDHURI, D.K. Heat shock response: hsp70 in enviromental monitoring. Journal of Biochemical and
Molecular Toxicology, v. 17(5), p. 249-254, 2003a.
MUKHOPADHYAY, I.; SAXENA, D.K.; BAJPAI, V.K.; CHOWDHURI, D.K. Argenome oil induced cellular damage in the reproductive tissues of transgenic
Drosophila melanogaster: Protective role of 70kDa heat shock protein. Journal of Biochemical and Molecular Toxicology, v. 17(4), p. 223-234, 2003b.
NASS, M.M.K. Differential effects of ethidium bromide on mitochondrial and nuclear DNA synthesis in vivo in cultured mammalian cells. Exp. Cell. Res., v. 72, p. 522, 1972.
&DStWXOR,9&DStWXOR,9&DStWXOR,9&DStWXOR,9115
NAZIR, A.; MUKHOPADHYAY, I.; SAXENA, D. K.; KAR CHOWDHURI, D. Chlorpyrifos induced hsp70 expression in transgenic Drosophila melanogaster (hsp70- lacZ) Bg9. Arch. Environ. Toxicol., v. 41, p. 443-449, 2001.
NAZIR, A.; MUKHOPADHYAY, I.; SAXENA, D.K.; CHOWDHURI, D.K. Evaluation of the No Observed Adverse Effect Level of Solvent Dimethyl Sulfoxide in
Drosophila melanogaster. Toxicology Mechanisms and Methods, v.13 (2), 2003.
NISHIWAKI, H.; MIURA, M.; IMAI, K.; OHMO, R.. KASWASHUMA, K.; EZAKI, K.; UCEDA, R.; YOSHIKAWA, H.; NAGATA, K.; TAKEYAMA, H.; YAMADA, K.
Cancer Research, v.34, p. 2699, 1974.
OUCHI, R.Y.; MANZATO, A.J.; CERON, C.R.; BONILLA-RODRIGUEZ, G.O. Evaluation of the effects of a single exposure to ethidium bromide in Drosophila
melanogaster (Diptera-Drosophilidae). Bull of Environ Contam Toxicol, v. 78, p. 489-
493, 2007.
ORR, W. C; SOHAL, R. S. Extension of Life-Span by Overexpression of Superoxide Dismutase and Catalase in Drosophila melanogaster. Science, v. 263, p. 1128-1130, 1994.
PACK, G.R.; LOEW, G. Origins of the specificity in the intercalation of ethidium into nucleic acids. A theoretical analysis. Biochim Biophys Acta, v. 519, p. 163-172, 1978.
PEÑA, A.; CHÁVEZ, E.; CÁRABEZ, A.; DE GÓMEZ-PUYOU, M.T. The metabolic effects and uptake of ethidium bromide by rat liver mitochondria. Arch. Biochem
Biophys, v. 180, p. 522, 1977.
PERLMAN, P.S.; MAHLER, H.R. Molecular consequences of ethidium bromide mutagenesis. Nature New Biol, v. 231, p. 12-16, 1971.
PFAFFL M.W. A new mathematical model for relative quantification in real-time RT- PCR. Nucleic Acids Research, v. 9, p. 29-45, 2001.
de POMERAI, D. Heat-shock proteins as biomarkers of pollution. Hum. Exp. Toxicol. v. 15(4), p. 279-85, 1996.
RICHMOND, R.C.; GILBERT, D.G.; SHEEHAN, K.B.;GROMKO, M.H.; BUTTERWORTH, F.M. Esterase 6 and reproduction in Drosophila melanogaster.
Science, v. 207, p. 1483-5, 1980.
SAMBROOK, J.; FRITSCH, E.F.; MANIATIS T. Molecular Cloning A Laboratory Manual, 2nd ed. Cold Spring Harbor, 1989.
SANDERS, B. M.; MARTIN L. S. Stress proteins as biomarkers of contaminant exposure in archived enviromental samples. Sci Total Environ, v. 139-141, p. 459-70, 1993.
&DStWXOR,9&DStWXOR,9&DStWXOR,9&DStWXOR,9116
SLONISMSKI, P.P.; PERRODIN, G.; GROFTH, J.H. Ethidium bromide induced mutation of yeast mitochondria: complete transformation of cells into respiratory deficient non-chromosomal “petites”. Biochem Biophys Res Comm, v. 30, p. 232-239, 1968.
TIBURI, M.; REGULY, M.L. SCHWATSMANN, G.; CUNHA, K.S.; LEHMANN, M.; ANDRADE, H.H.R. Comparative genotoxic effect of vincristine, vinblastine, and vinorelbine in somatic cells of Drosophila melanogaster. Mutat res, v. 519, p. 141-149, 2002.
VARO, I.; SERRANO, R.; PITARCH, E.; AMAT, F.; LOPEZ, F.J.; NAVARRO, J.C. Bioaccumulation of chlorpyrifos through an experimental food chain: study of protein HSP70 as biomarker of sublethal stress in fish. Arch Environ Contam Toxicol., v. 42(2), p. 229-35, 2002
VALKO, M.; Rhodes, C.J.; Moncol, J.; Izakovic, M.; Mazur, M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chemico-Biological Interactions, v. 160, p. 1–40, 2006.
VACQUIER, V.D.; BRACHET, J. Chromosomal abnormalities resulting from ethidium bromide treatment. Nature, v. 222, p. 193-195, 1969.
WU, G.; Fang, Y-Z.; Yang, S.; Lupton, J.R.; Turner, N.D. Glutathione Metabolism and Its Implications for Health. J. Nutr., v. 134, p. 489-492, 2004.