• No results found

Generation of knock-out cells by using CRISPR/Cas9

In document A study of TRIM32 self-ubiquitination (sider 58-64)

3. Materials and Methods

3.3. Generation of knock-out cells by using CRISPR/Cas9

Como sugestões de ampliar este estudo e para trabalhos futuros propõem-se:

 Avaliar melhor o método de aplicação dos filmes e testar outros métodos de aplicação.

 Caracterizar os nanocompósito por MEV, para explicar melhor como se encontra a dispersão das nanopartículas na matriz polimérica.

ANEXOS

Anexo I: Espectros de UV-vis dos nanocompósitos com (a) 0,5 %, (b) 1%, (c) 1,5% e (d) 2% de TiO2 antes e após envelhecimento UV por 180 minutos.

400 500 600 700 800 900 -0,005 0,000 0,005 0,010 0,015 0,020 0,025 0,030 Ab so rb ân cia (% ) Comprimento de Onda (nm) 0 min. 180 min. 0 2 4 6 8 10 0 2 4 6 8 10 (a) 400 500 600 700 800 900 0,000 0,005 0,010 0,015 0,020 0,025 0,030 0,035 0,040 Ab so rb ân cia (% ) Comprimento de Onda (nm) 0 min. 180 min. 0 2 4 6 8 10 0 2 4 6 8 10 (b)

400 500 600 700 800 900 0,000 0,005 0,010 0,015 0,020 0,025 0,030 0,035 0,040 Ab so rb ân cia (% ) Comprimento de Onda (nm) 0 min. 180 min. 0 2 4 6 8 10 0 2 4 6 8 10 (c) 400 500 600 700 800 900 0,000 0,005 0,010 0,015 0,020 0,025 0,030 0,035 Ab so rb ân cia (% ) Comprimento de Onda (nm) 0 min. 180 min. 0 2 4 6 8 10 0 2 4 6 8 10 (d)

Capítulo VI

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In document A study of TRIM32 self-ubiquitination (sider 58-64)