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Fungal associates of the tree-killing bark beetle, Ips typographus, vary in virulence, ability to degrade conifer phenolics and influence bark beetle tunneling behavior

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Academic year: 2022

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(1)

0 40 80 120 160 200

Phloem lesion leng th (mm)

a a

ab

bc bc bc bc

de cd de de e

Gp Ge Ep Op Ob Control

(2)
(3)

0 10 20 30

Piceid

Isorhaponn Astringin

Conidendrin Nortachelogenin Isolariciresinol Matairesinol

Secoisolariciresinol Lariciresinol

Pinoresinol Coniferyl alcohol

Slbene glucosides

Taxifolin glucoside Quercen glucoside Proanthocyanidin dimers Gallocatechin

Catechin 0

5 10 15 20 25 30

0 10 20 30 40

mg/g dry spruce ssue

Lignans Flavonoids

a ab

ab

bc bc

bc

c

a ab

abc bcd abcd

cd

d

a

ab ab ab

b b b

Gp1 Gp2 Ge Ep Op Ob Control

(4)

Rhapongenin Piceatannol Resveratrol

0 1 2 3 4

mg/g dry spruce ssue

a

ab

ab

ab ab ab

b

Gp1 Gp2 Ge Ep Op Ob Control

(5)

0 4 8 12 16 20

Growth rate (mm day

-1

)

a b

c

d

e

0 1 2 3 4 5

a b

c

d

e

Spruce bark agar

Minimal medium with glucose

Gp Ge Ep Op Ob

(6)

Catechin (1mg/ml)

w/o Catechin No choice 02 0 4 0 60 80 100 Choice (%)

N=4, P =0.040

7% phloem agar

Catechin+ Ep (1mg/ml)

w/o Catechin No choice 02 0 4 0 60 80 100

N=4, P =0.002

7% phloem agar

A B

(7)

Control Ep Ge Ob No choice

Choice (%)

Control Ep Gp Op

801006040200

801006040200

No choice

Choice (%)

Ep Control

Ge Ob

A

B C

N=5, P=0.15 N=4, P=0.07

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