Time scale: 22 25 26 Hours after slaughter
A1 (Non-crowded)
A2 (Non-crowded)
Con B (crowded)
TTA B (crowded)
A2 (Non-crowded)
A1 (Non-crowded)
Arg B (crowded)
A2 (Non-crowded)
A1 (Non-crowded)
Glu
B (crowded) A2 (Non-crowded)
A1 (Non-crowded)
Production sampling: analysis of 10 fish from each net pen during each sampling from April to September.
Date (22, 25 and 26) of June is the time for shifting TTA diet to Control for net pen number 9, 6 and 1 respectively. Fish were fed with TTA diet until they reached 0.2% of body weight.
Pre-rigor fillets analysis: analysis of 6 fish (non-crowded) and 3 fish (crowded) from each net pen in the last sampling time (15-17th of September).
A1=measurements of muscle pH and collection of muscle samples from right fillets of non-crowded fish.
A2= measurements of fillet length, color and gaping from left fillets of non-crowded fish.
B= sampling of muscle pH and collection of muscles from right fillets and measurements of fillet length, color and gaping from left fillets of crowded fish.
Total biomass
Condition factor
Liver weight
Heart weight
Pigment
Fat content
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Net pens
1 3 6 9 12
Analyzed parameters in each production sampling
24 48 72
Fillet length (1, 3, 6, 9, 12, 24, 48 and 72 hours for Non-crowded fish and 1, 6, 12, 24 and 48 hours for crowded fish) Fillet color (1, 6, 12, 24 and 48 hours for Non- crowded fish and crowded fish)
Gaping (9 and 24 hours for Non-crowded fish and crowded fish) Muscle pH (1, 6, 12, 24, 48 and 72 for Non- crowded fish and 1, 12 and 48 for crowded fish) Muscle samples (1, 6, 12, 24, 48 and 72 for Non- crowded fish and 1, 12 and 48 for crowded fish)
Analyzed parameters of pre- rigor fillets
Diet
Sampling 1 26-27. May. 2009
Sampling 3 29. July. 2009
Sampling 4 15-17. Sep. 2009 Starting point of project (Sampling 0)
15. Apr.2009
Sampling 2 23-24. June. 2009